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The Development of T Lymphocytes in Fetal Thymus Organ Culture

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Book cover Basic Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 946))

Abstract

Fetal thymus organ culture (FTOC) is a unique and powerful culture system that allows intrathymic T-lymphocyte development in vitro. T-cell development in FTOC well represents fetal thymocyte development in vivo. Here we describe the basic method for FTOC as well as several related techniques, including reconstitution of thymus lobes with T-lymphoid progenitor cells, high-oxygen submersion culture, reaggregation thymus organ culture, retrovirus-mediated gene transfer to developing thymocytes in FTOC, and coculture of progenitor cells with OP9-DL1 cells.

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Author information

Authors and Affiliations

  1. Division of Experimental Immunology, Institute for Genome Research, University of Tokushima, Tokushima, Japan

    Takeshi Nitta & Izumi Ohigashi

  2. Division of Experimental Immunology, Institute for Genome Research, The University of Tokushima, Tokushima, Japan

    Yousuke Takahama

Authors
  1. Takeshi Nitta
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  2. Izumi Ohigashi
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  3. Yousuke Takahama
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Corresponding author

Correspondence to Yousuke Takahama .

Editor information

Editors and Affiliations

  1. , Experimental Therapeutics, BC Cancer Agency, West 10th Ave. 675, Vancouver, V5Z 1L3, British Columbia, Canada

    Cheryl D. Helgason

  2. STEMCELL Technologies, Inc., W. Seventh Avenue 570, Vancouver, V5Z 1B3, British Columbia, Canada

    Cindy L. Miller

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Nitta, T., Ohigashi, I., Takahama, Y. (2013). The Development of T Lymphocytes in Fetal Thymus Organ Culture. In: Helgason, C., Miller, C. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology, vol 946. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-128-8_6

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  • DOI: https://doi.org/10.1007/978-1-62703-128-8_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-127-1

  • Online ISBN: 978-1-62703-128-8

  • eBook Packages: Springer Protocols

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