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Epithelial Cell Culture Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Scott H. Randell

    1. Cystic Fibrosis/Pulmonary Research Ctr., University of North Carolina, Chapel Hill, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. M. Leslie Fulcher

    1. Cystic Fibrosis/Pulmonary Research Ctr., The University of North Carolina, Chapel Hill, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 945)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Progress Towards Drosophila Epithelial Cell Culture

    • Amanda Simcox
    Pages 1-11

  3. Culture of Choroid Plexus Epithelial Cells and In Vitro Model of Blood–CSF Barrier

    • Andrew D. Monnot, Wei Zheng
    Pages 13-29

  4. Methods for Culture of Human Corneal and Conjunctival Epithelia

    • Sandra J. Spurr-Michaud, Ilene K. Gipson
    Pages 31-43

  5. The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eye

    • Timothy A. Blenkinsop, Enrique Salero, Jeffrey H. Stern, Sally Temple
    Pages 45-65

  6. Oral and Pharyngeal Epithelial Keratinocyte Culture

    • Kantima Leelahavanichkul, J. Silvio Gutkind
    Pages 67-79

  7. Primary Culture of the Human Olfactory Neuroepithelium

    • Nancy E. Rawson, Mehmet Hakan Ozdener
    Pages 81-93

  8. Primary Culture of Mammalian Taste Epithelium

    • Mehmet Hakan Ozdener, Nancy E. Rawson
    Pages 95-107

  9. Human Nasal and Tracheo-Bronchial Respiratory Epithelial Cell Culture

    • M. Leslie Fulcher, Scott H. Randell
    Pages 109-121

  10. Culture and Differentiation of Mouse Tracheal Epithelial Cells

    • Yingjian You, Steven L. Brody
    Pages 123-143

  11. Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs

    • Robert F. Gonzalez, Leland G. Dobbs
    Pages 145-159

  12. Classical Human Epidermal Keratinocyte Cell Culture

    • Cathy Rasmussen, Christina Thomas-Virnig, B. Lynn Allen-Hoffmann
    Pages 161-175

  13. Mouse Epidermal Keratinocyte Culture

    • Luowei Li
    Pages 177-191

  14. Three-Dimensional Culture of Human Breast Epithelial Cells: The How and the Why

    • Pierre-Alexandre Vidi, Mina J. Bissell, Sophie A. Lelièvre
    Pages 193-219

  15. Three-Dimensional Cultures of Mouse Mammary Epithelial Cells

    • Rana Mroue, Mina J. Bissell
    Pages 221-250

  16. Isolation, Characterization, and Reaggregate Culture of Thymic Epithelial Cells

    • Natalie Seach, Maree Hammett, Ann Chidgey
    Pages 251-272

  17. Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development

    • Akihide Kamiya, Hiromitsu Nakauchi
    Pages 273-286

  18. Long-Term Culture and Coculture of Primary Rat and Human Hepatocytes

    • Maria Shulman, Yaakov Nahmias
    Pages 287-302

  19. Tissue Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidney Disease

    • Yasuni Nakanuma, Yasunori Sato, Kenichi Harada
    Pages 303-318

  20. Primary Mouse Small Intestinal Epithelial Cell Cultures

    • Toshiro Sato, Hans Clevers
    Pages 319-328
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Keywords

About this book

Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few. Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science.

Editors and Affiliations

  • Cystic Fibrosis/Pulmonary Research Ctr., University of North Carolina, Chapel Hill, USA

    Scott H. Randell

  • Cystic Fibrosis/Pulmonary Research Ctr., The University of North Carolina, Chapel Hill, USA

    M. Leslie Fulcher

Bibliographic Information

  • Book Title: Epithelial Cell Culture Protocols

  • Editors: Scott H. Randell, M. Leslie Fulcher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-125-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-124-0Published: 25 October 2012

  • Softcover ISBN: 978-1-4939-5679-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-125-7Published: 25 October 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 469

  • Topics: Cell Biology, Cell Culture

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