Abstract
Phytoplasmas are routinely detected by nucleic acid-based techniques. These approaches rely on enriched phytoplasma DNA extracts of good quality, following labor intensive and time-consuming purification protocols. Here we describe a very rapid, specific, sensitive, and reliable method for flavescence dorée phytoplasma detection, based on real-time Taqman® reverse transcription-PCR of the 16S rRNA. The protocol is particularly useful for large-scale screening of vineyards and nurseries, pathogen surveys, and field epidemiological studies.
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Acknowledgements
P. M. was supported by the grant “Studi sui fattori che favoriscono le epidemie di flavescenza dorata in Piemonte e loro superamento,” sub-project C, from Regione Piemonte, Italy (Report number 12851).
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Margaria, P., Palmano, S. (2013). Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions. In: Dickinson, M., Hodgetts, J. (eds) Phytoplasma. Methods in Molecular Biology, vol 938. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-089-2_24
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DOI: https://doi.org/10.1007/978-1-62703-089-2_24
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Publisher Name: Humana Press, Totowa, NJ
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