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In Vitro Spermatogenesis Using an Organ Culture Technique

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Spermatogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 927))

Abstract

Research on in vitro spermatogenesis has a long history and remained to be an unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm from primitive spermatogonia using an organ culture method. The fertility of the sperm or haploid spermatids was demonstrated by microinsemination. This organ culture technique uses the classical air–liquid interphase method and is based on conditions extensively examined by Steinbergers in 1960s. Among adaptations in the new culture system, application of serum-free media was the most important. The system is very simple and easy to follow.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas, “Regulatory Mechanism of Gamete Stem Cells” (#20116005); a Grant-in-Aid for Scientific Research (C) (#21592080) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan; a grant from the Yokohama Foundation for Advancement of Medical Science; and a grant for Research and Development Project II (No.S2116) of Yokohama City University, Japan, to TO.

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Authors and Affiliations

  1. Department of Urology, Yokohama City University Graduate School of Medicine, Kanazawa-ku, Yokohama, Japan

    Tetsuhiro Yokonishi, Kumiko Katagiri & Takehiko Ogawa

  2. Department of Urology, Graduate School of Medicine, Yokohama City University, Yokohama, Japan

    Takuya Sato

Authors
  1. Tetsuhiro Yokonishi
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  2. Takuya Sato
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  3. Kumiko Katagiri
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  4. Takehiko Ogawa
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Corresponding author

Correspondence to Takehiko Ogawa .

Editor information

Editors and Affiliations

  1. Andrology and IVF Laboratories, Department of Surgery (Urology), University of Utah School of Medicine, Arapeen Dr. 675, Salt Lake City, 84108, Utah, USA

    Douglas T. Carrell

  2. , Andrology and IVF Laboratories, University of Utah School of Medicine, Arapeen Way 675, Salt Lake City, 84108, Utah, USA

    Kenneth I. Aston

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Yokonishi, T., Sato, T., Katagiri, K., Ogawa, T. (2013). In Vitro Spermatogenesis Using an Organ Culture Technique. In: Carrell, D., Aston, K. (eds) Spermatogenesis. Methods in Molecular Biology, vol 927. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-038-0_41

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  • DOI: https://doi.org/10.1007/978-1-62703-038-0_41

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-037-3

  • Online ISBN: 978-1-62703-038-0

  • eBook Packages: Springer Protocols

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