Abstract
Salinity and oxidative stress can transiently elevate cytosolic free Ca2+ ([Ca2+]cyt) of plant cells. The [Ca2+]cyt increase may be part of a signaling cascade or cell death, depending on cell type, the magnitude and the duration of stress exposure. Several approaches for determining [Ca2+]cyt responses are available to plant biologists, but some require highly specialized equipment. Here we describe protocols for using aequorin as a standard [Ca2+]cyt reporter, with output detected with a plate-reader luminometer.
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Acknowledgements
We thank Vadim Demidchik, Jenny Mortimer, Zhonglin Shang for all their efforts in establishing these protocols and Alex Webb for the aequorin construct. This work was funded by the BBSRC, the Frank Smart Trust and the Brookes Trust.
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Laohavisit, A., Colaço, R., Davies, J. (2012). Cytosolic Ca2+ Determinations in Studying Plant Responses to Salinity and Oxidative Stress. In: Shabala, S., Cuin, T. (eds) Plant Salt Tolerance. Methods in Molecular Biology, vol 913. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-986-0_10
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DOI: https://doi.org/10.1007/978-1-61779-986-0_10
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