Abstract
Gonorrhoea is no longer an easily treatable ailment but rather is now a challenging disease in terms of antimicrobial resistance (AMR) with treatment options rapidly diminishing. The causative agent of gonorrhoea, Neisseria gonorrhoeae, has managed to develop resistance to almost every single drug used against it with the sole exception of extended spectrum cephalosporins. The situation is further exacerbated by the fact that not only are the rates of gonococcal infections on a steady rise globally, but tracking AMR is being undermined by the growing popularity of molecular methods at the expense of traditional bacterial culture in diagnostic laboratories. Recently, concerns have been raised over the emergence of a multi-resistant gonococci and the potential for untreatable gonorrhoea. Maintaining optimal epidemiological surveillance of gonococcal AMR remains an important aspect of gonorrhoea control. The development of molecular tools for tracking AMR in N. gonorrhoeae has the potential to further enhance such surveillance. In this chapter, we discuss nucleic acid amplification-based detection of AMR in gonorrhoea with a particular emphasis on chromosomal-mediated resistance to beta-lactam antibiotics.
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References
Centers for Disease Control and Prevention (2003) Sexually transmitted disease surveillance, 2003. US Department of Health and Human Services, CDC, Atlanta, GA
Tapsall J (2006) Antibiotic resistance in Neisseria gonorrhoeae is diminishing available treatment options for gonorrhea: some possible remedies. Expert Rev Anti Infect Ther 4:619–628
Tapsall J (2009) Implications of current recommendations for third-generation cephalosporin use in the WHO Western Pacific region following the emergence of multi-resistant gonococci. Sex Transm Infect 85:256–258
Barry PM, Klausner JD (2009) The use of cephalosporins for gonorrhoea: the problem of impending resistance. Exp Opin Pharmacother 10:555–577
Vernel-Pauillac F, Nandi S, Nicholas RA et al (2008) Genotyping as a tool for antibiotic resistance surveillance of Neisseria gonorrhoeae in New Caledonia: evidence of a novel genotype associated with reduced penicillin susceptibility. Antimicrob Agents Chemother 52:3293–3300
Zhao S, Duncan M, Tomberg J et al (2009) Genetics of chromosomally mediated intermediate resistance to ceftriaxone and cefixime in Neisseria gonorrhoeae. Antimicrob Agents Chemother 53:3744–3751
Tomberg J, Unemo M, Davies C et al (2010) Molecular and structural analysis of mosaic variants of penicillin-binding protein 2 conferring decreased susceptibility to expanded-spectrum cephalosporins in Neisseria gonorrhoeae: role of epistatic mutations. Biochemistry 49:8062–8070
Ito M, Deguchi T, Mizutani KS et al (2005) Emergence and spread of Neisseria gonorrhoeae clinical isolates harboring mosaic-like structure of penicillin-binding protein 2 in Central Japan. Antimicrob Agents Chemother 49:137–143
Whiley DM, Limnios EA, Ray S et al (2007) Diversity of penA alterations and subtypes in Neisseria gonorrhoeae strains from Sydney, Australia, that are less susceptible to ceftriaxone. Antimicrob Agents Chemother 51:3111–3116
Kugelman G, Tapsall JW, Goire N et al (2009) Simple, rapid, and inexpensive detection of Neisseria gonorrhoeae resistance mechanisms using heat-denatured isolates and SYBR green-based real-time PCR. Antimicrob Agents Chemother 53:4211–4216
Liew M, Pryor R, Palais R et al (2004) Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin Chem 50:1156–1164
Whiley DM, Goire N, Lambert SB et al (2010) Reduced susceptibility to ceftriaxone in Neisseria gonorrhoeae is associated with mutations G542S, P551S and P551L in the gonococcal penicillin-binding protein 2. J Antimicrob Chemother 65:1615–1618
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Goire, N., Sloots, T.P., Nissen, M.D., Whiley, D.M. (2012). Protocol for the Molecular Detection of Antibiotic Resistance Mechanisms in Neisseria gonorrhoeae . In: MacKenzie, C., Henrich, B. (eds) Diagnosis of Sexually Transmitted Diseases. Methods in Molecular Biology, vol 903. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-937-2_22
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DOI: https://doi.org/10.1007/978-1-61779-937-2_22
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