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Visualization Techniques

From Immunohistochemistry to Magnetic Resonance Imaging

  • Book
  • © 2012

Overview

Editors:
  1. Emilio Badoer

    1. School of Medical Sciences, Pharmaceutical Sciences, RMIT University, Bundoora, Australia

    View editor publications

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  • Covers the top methods of visualizing neurochemistry
  • Features step-by-step procedures to guide readers through key experiments
  • Includes key tips and detailed implementation advice to ensure quality results

Part of the book series: Neuromethods (NM, volume 70)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Multiple Immunohistochemical Labelling of Peripheral Neurons

    • Ian L. Gibbins
    Pages 1-29

  3. Combined In Situ Hybridization and Immunohistochemistry in Rat Brain Tissue Using Digoxigenin-Labeled Riboprobes

    • Natasha N. Kumar, Belinda R. Bowman, Ann K. Goodchild
    Pages 31-52

  4. In Situ Hybridization Within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence

    • Dominic Bastien, Steve Lacroix
    Pages 53-70

  5. Visualizing GABAB Receptor Internalization and Intracellular Trafficking

    • Paola Ramoino, Paolo Bianchini, Alberto Diaspro, Cesare Usai
    Pages 71-95

  6. Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualise Insulin Action

    • James G. Burchfield, Jamie A. Lopez, William E. Hughes
    Pages 97-109

  7. Live-Cell Quantification of Mitochondrial Functional Parameters

    • Marco Nooteboom, Marleen Forkink, Peter H. G. M. Willems, Werner J. H. Koopman
    Pages 111-127

  8. Functional Imaging Using Two-Photon Microscopy in Living Tissue

    • Ivo Vanzetta, Thomas Deneux, Attila Kaszás, Gergely Katona, Balazs Rozsa
    Pages 129-164

  9. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

    • Audrey Bonnan, Benjamin Grewe, Andreas Frick
    Pages 165-187

  10. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

    • Ruth L. Stornetta
    Pages 189-205

  11. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

    • Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum et al.
    Pages 207-223

  12. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

    • J. Patrick Card, Lynn W. Enquist
    Pages 225-268

  13. Visualisation of Thermal Changes in Freely Moving Animals

    • Daniel M. L. Vianna, Pascal Carrive
    Pages 269-281

  14. Perfusion Magnetic Resonance Imaging Quantification in the Brain

    • Fernando Calamante
    Pages 283-312

  15. Back Matter

    Pages 313-315
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Keywords

About this book

Visualization of chemicals in tissues has seen incredible advances in the past several years. Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging provides practical advice from experts in the field as well as an excellent overview of some of the most important recent advances in visualization.  This timely volume explores topics from immunohistochemistry for multiple neurochemicals, detecting expression levels of neurochemicals, following cellular processes and ionic movement, identifying polysynaptic pathways subserving physiological responses to identifying functional changes in vivo. Written for the popular Neuromethods series, this work includes the kind of detailed description and implementation advice that is crucial for getting optimal results in the lab.

 

Meticulous and concise, Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging will prove invaluable for scientists seeking to gain a greater understanding of the practical skills, strengths, and pitfalls that these wonderful and exciting visualization techniques provide.

Reviews

From the reviews:

“This is an excellent overview of advances in visualization techniques in neuroscience, particularly at the molecular level. The book is written for neuroscientists, basic neuroresearchers, and students of medical science. It is also appropriate for clinical and academic specialists in neurobiology, neuropharmacology, neurology, neuro-oncology, neuroradiology, and neurosurgery. … It is an inspiration for investigators interested in furthering their knowledge of the visualization of the nervous system not only in its entirety, but also at the neuronal level.” (Ramsis Farid Ghaly, Doody’s Book Reviews, August, 2012)

Editors and Affiliations

  • School of Medical Sciences, Pharmaceutical Sciences, RMIT University, Bundoora, Australia

    Emilio Badoer

Bibliographic Information

  • Book Title: Visualization Techniques

  • Book Subtitle: From Immunohistochemistry to Magnetic Resonance Imaging

  • Editors: Emilio Badoer

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-61779-897-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-896-2Published: 08 June 2012

  • Softcover ISBN: 978-1-4939-5840-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-897-9Published: 07 June 2012

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 316

  • Topics: Neurosciences, Neurochemistry, Diagnostic Radiology

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