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Protein Electrophoresis pp 111–118Cite as

Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 869))

Abstract

Very large proteins (subunit sizes >200 kDa) are difficult to electrophoretically separate on polyacrylamide gels. A SDS vertical agarose gel system has been developed that has vastly improved resolving power for very large proteins. Proteins with molecular masses between 200 and 4,000 kDa can be clearly separated. Inclusion of a reducing agent in the upper reservoir buffer has been found to be a key technical procedure for obtaining optimum resolution.

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Acknowledgments

This work was supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison, and from grants (MLG-NIH HL77196 and Hatch NC1131).

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Authors and Affiliations

  1. University of Wisconsin-Madison, Madison, WI, USA

    Marion L. Greaser

  2. University of Illinois at Chicago, Chicago, IL, USA

    Chad M. Warren

Authors
  1. Marion L. Greaser
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  2. Chad M. Warren
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Corresponding author

Correspondence to Marion L. Greaser .

Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, Univ. of Oklahoma Health Sciences Center, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    R. Hal Scofield

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Greaser, M.L., Warren, C.M. (2012). Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_10

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

  • eBook Packages: Springer Protocols

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