Abstract
Careful examination of how explants react to experimental conditions is the first step of a successful research program. Photographing the specimen is an extremely important method that can be used to document changes of an explant throughout an experiment. This article serves to draw attention to the utility of macroscopic and microscopic examinations in the study of in vitro development, and details some useful methods in the study of the cultured explants.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wetmore RH, Wardlaw CW (1951) Experimental morphogenesis in vascular plants. Annu Rev Plant Physiol 2:269–292
Steeves TA, Sussex IM (1989) Patterns in plant development. Cambridge University Press, Cambridge
Yeung EC (1999) The use of histology in the study of plant tissue culture systems – some practical comments. In Vitro Cell Dev Biol Plant 35:137–143
Yeung EC, Sexena PK (2005) Histological techniques. In: Jain SM, Gupta PK (eds) Protocol for somatic embryogenesis in woody plants. Springer, Dordrecht, pp 517–537
Campton ME, Mize CW (1999) Statistical considerations for in vitro research: I – birth of an idea to collecting data. In Vitro Cell Dev Biol Plant 35:115–121
Mize CW, Koehler KJ, Compton ME (1999) Statistical considerations for in vitro research: II – data to presentation. In Vitro Cell Dev Biol Plant 35:122–126
Wong CK, Yeung EC, Wong HYS (1992) Multiple shoot formation from mature cotyledonary node of the bean. Phaseolus coccineus L. I. In vitro culture technique. Chin Agron J 2:155–167
Yeung EC, Aitken J, Biondi S, Thorpe TA (1981) Shoot histogenesis in cotyledon explants of radiata pine. Bot Gaz 142:494–501
Ogita S, Yeung EC, Sasamota H (2004) Histological analysis in shoot organogenesis from hypocotyls explants of Kandelia candel (Rhizophoraceae). J Plant Res 117:457–464
Bassuner BM, Lam R, Lukowitz W, Yeung EC (2007) Auxin and root initiation in somatic embryos of Arabidopsis. Plant Cell Rep 26:1–11
Nickle TC, Yeung EC (1993) Failure to establish a functional shoot meristem may be a cause of conversion failure in somatic embryos of Daucus carota (Apiaceae). Am J Bot 80:1284–1291
Yeung EC, Stasolla C (2001) Somatic embryogenesis – apical meristems and embryo conversion. Korean J Plant Tissue Cult 27:299–307
Thomson DJ, Bradbury S (1987) An introduction to photomicrography. Oxford University Press, New York
O’Brien TP, McCully ME (1981) The study of plant structure: principles and selected methods. Termarcarphi, Melbourne
Ruzin SE (1999) Plant microtechnique and microscopy. Oxford University Press, New York
O’Brien TP, Feder N, McCully ME (1964) Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma 59:368–373
Peterson RL, Peterson CA, Melville LH (2008) Teaching plant anatomy. NRC, Canada
Jensen WA (1962) Botanical histochemistry. Freeman, San Francisco
Gahan PB (1984) Plant histochemistry and cytochemistry – an introduction. Academic, London
Yeung EC (1998) A beginner’s guide to the study of plant structure. In: Karcher SJ (ed) Tested studies for laboratory teaching, vol 19. Proceeding of the 19th workshop/conference of the Association for Biology Laboratory Education (ABLE). Purdue University, Lafayette, IN, pp 125–141
Acknowledgments
I am grateful to the Natural Sciences and Engineering Research Council of Canada for the continuous funding of my research program.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Yeung, E.C. (2012). The Study of In Vitro Development in Plants: General Approaches and Photography. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_8
Download citation
DOI: https://doi.org/10.1007/978-1-61779-818-4_8
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-817-7
Online ISBN: 978-1-61779-818-4
eBook Packages: Springer Protocols