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Fluorescence-Based Biosensors

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Spectroscopic Methods of Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 875))

Abstract

The field of optical sensors has been a growing research area over the last three decades. A wide range of books and review articles has been published by experts in the field who have highlighted the advantages of optical sensing over other transduction methods. Fluorescence is by far the method most often applied and comes in a variety of schemes. Nowadays, one of the most common approaches in the field of optical biosensors is to combine the high sensitivity of fluorescence detection in combination with the high selectivity provided by ligand-binding proteins.

In this chapter we deal with reviewing our recent results on the implementation of fluorescence-based sensors for monitoring environmentally hazardous gas molecules (e.g. nitric oxide, hydrogen sulfide). Reflectivity-based sensors, fluorescence correlation spectroscopy-based (FCS) systems, and sensors relying on the enhanced fluorescence emission on silver island films (SIFs) coupled to the total internal reflection fluorescence mode (TIRF) for the detection of gliadin and other prolamines considered toxic for celiac patients are also discussed herein.

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Authors and Affiliations

  1. Department of Chemistry, University of Salerno, Salerno, Italy

    Maria Strianese & Claudio Pellecchia

  2. Laboratory for Molecular Sensing, IBP-CNR, Naples, Italy

    Maria Staiano, Giuseppe Ruggiero, Tullio Labella & Sabato D’Auria

Authors
  1. Maria Strianese
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  2. Maria Staiano
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  3. Giuseppe Ruggiero
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  4. Tullio Labella
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  5. Claudio Pellecchia
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  6. Sabato D’Auria
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Corresponding author

Correspondence to Sabato D’Auria .

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Editors and Affiliations

  1. Dept. Biochemistry & Molecular Biology, University of Texas Medical Branch, University Blvd. 301, Galveston, 77555-1053, Texas, USA

    Wlodek M. Bujalowski

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Strianese, M., Staiano, M., Ruggiero, G., Labella, T., Pellecchia, C., D’Auria, S. (2012). Fluorescence-Based Biosensors. In: Bujalowski, W. (eds) Spectroscopic Methods of Analysis. Methods in Molecular Biology, vol 875. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-806-1_9

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  • DOI: https://doi.org/10.1007/978-1-61779-806-1_9

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  • Publisher Name: Humana Press, Totowa, NJ

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