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Principles for Derivation of Human Embryonic Stem Cells

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Human Embryonic Stem Cells Handbook

Part of the book series: Methods in Molecular Biology ((MIMB,volume 873))

Abstract

This chapter describes the principles for derivation and maintenance of human embryonic stem cells. Detailed protocols are outlined and researchers who are generally skilled in mammalian cell culture should be able to repeat the processes successfully. Further, the protocols are intended for scientists who do not have access to advanced IVF equipment and therefore cannot perform, e.g. assisted hatching. In addition to derivation, we also discuss characterisation and banking of hES cells.

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Author information

Authors and Affiliations

  1. Cellartis AB, Dundee, UK

    Mikael C. O. Englund, Catharina Ellerström, Katarina Andersson, Karin Noaksson & Johan Hyllner

Authors
  1. Mikael C. O. Englund
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  2. Catharina Ellerström
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  3. Katarina Andersson
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  4. Karin Noaksson
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  5. Johan Hyllner
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Corresponding author

Correspondence to Mikael C. O. Englund .

Editor information

Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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© 2012 Springer Science+Business Media, LLC

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Englund, M.C.O., Ellerström, C., Andersson, K., Noaksson, K., Hyllner, J. (2012). Principles for Derivation of Human Embryonic Stem Cells. In: Turksen, K. (eds) Human Embryonic Stem Cells Handbook. Methods in Molecular Biology, vol 873. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-794-1_4

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  • DOI: https://doi.org/10.1007/978-1-61779-794-1_4

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-793-4

  • Online ISBN: 978-1-61779-794-1

  • eBook Packages: Springer Protocols

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