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Extraction and Isolation of Saponins

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Natural Products Isolation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 864))

Abstract

Due to their special structural features, extraction and isolation of saponins poses a serious challenge. Conventional methods have been explored as well as the recent, relatively greener, efficient, solvent-economic, time-saving, newer methods of extraction. Both traditional and recent methods of isolation are also discussed. Finally, examples are given involving both conventional and newer methods of extraction and isolation. Though in general it is difficult to use a single technique for isolation of saponins, recent literature work seems to point to the fact that high speed counter-current separation coupled to evaporative light scattering detector (ELSD) gives superior separation. The ELSD appears to have circumvented the long-standing problem of saponin detection as most of these do not have a chromophore, and hence making UV detection only nonspecific and at range 200–210 nm.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Botswana, Gaborone, Botswana

    Runner R. T. Majinda

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  1. Runner R. T. Majinda
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Correspondence to Runner R. T. Majinda .

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Editors and Affiliations

  1. School of Applied Sciences, Department of Pharmacy, University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY, United Kingdom

    Satyajit D. Sarker

  2. , Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester, LE1 9BH, United Kingdom

    Lutfun Nahar

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© 2012 Springer Science+Business Media, LLC

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Majinda, R.R.T. (2012). Extraction and Isolation of Saponins. In: Sarker, S., Nahar, L. (eds) Natural Products Isolation. Methods in Molecular Biology, vol 864. Humana Press. https://doi.org/10.1007/978-1-61779-624-1_16

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  • DOI: https://doi.org/10.1007/978-1-61779-624-1_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-623-4

  • Online ISBN: 978-1-61779-624-1

  • eBook Packages: Springer Protocols

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