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Biolistic-Mediated Production of Transgenic Oil Palm

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Transgenic Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 847))

Abstract

The effectiveness of mannose (using phosphomannose isomerase [pmi] gene) as a positive selection agent to preferably allow the growth of transformed oil palm embryogenic calli was successfully evaluated. Using the above selection agent in combination with the previously optimized physical and biological parameters and the best constitutive promoter, oil palm embryogenic calli were transformed with pmi gene for producing transgenic plants. Bombarded embryogenic calli were exposed to embryogenic calli medium containing 30:0 g/L mannose to sucrose 3 weeks postbombardment. Selectively, proliferating embryogenic calli started to emerge around 6 months on the above selection medium. The proliferated embryogenic calli were individually isolated once they reached a specific size and regenerated to produce complete plantlets. The complete regenerated plantlets were evaluated for the presence of transgenes by PCR and Southern analyses.

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Acknowledgments

The author thanks the Director General of MPOB for permission to publish this chapter. Thanks are also due to Mdm. Fatimah Tahir of MPOB for her technical assistance and Dr. Omar Rasid for comments on the manuscript.

Author information

Authors and Affiliations

  1. Advanced Biotechnology and Breeding Centre, MPOB, Kajang, Selangor, Malaysia

    Ghulam Kadir Ahmad Parveez

  2. Advanced Biotechnology and Breeding Centre, MPOB, No 6, Persiaran Institusi, Kajang, Selangor, Malaysia

    Bohari Bahariah

Authors
  1. Ghulam Kadir Ahmad Parveez
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  2. Bohari Bahariah
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Corresponding author

Correspondence to Ghulam Kadir Ahmad Parveez .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Reading, Reading, Berkshire, RG6 6AS, United Kingdom

    Jim M. Dunwell

  2. Dept. of Agricultural Botany, School of Biological Sciences, University of Reading, Reading, United Kingdom

    Andy C. Wetten

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Parveez, G.K.A., Bahariah, B. (2012). Biolistic-Mediated Production of Transgenic Oil Palm. In: Dunwell, J., Wetten, A. (eds) Transgenic Plants. Methods in Molecular Biology, vol 847. Humana Press. https://doi.org/10.1007/978-1-61779-558-9_14

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  • DOI: https://doi.org/10.1007/978-1-61779-558-9_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-557-2

  • Online ISBN: 978-1-61779-558-9

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