Editors:
- Provides a selection of the key cellular,molecular, and biochemical techniques that are used in studying the many and varied functions of this fascinating cell
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 814)
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Table of contents (37 protocols)
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Front Matter
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Overview of Astrocyte Biology
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Front Matter
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Isolation and Culture of Astrocytes
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Front Matter
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Analysis of Astrocyte Functions In Vitro
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Front Matter
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About this book
Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma. Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS, including generation of neural stem cells, synaptogenesis, and regulation of the blood-brain barrier and neurovascular unit. Astrocytes: Methods and Protocols provides scientists with a comprehensive guide to many techniques used for astrocyte cell culture, as well as more specialized approaches for studying astrocyte functions, both in vitro and in vivo. Key cellular, molecular and biochemical techniques are used to study the many and varied functions of this fascinating cell. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Editors and Affiliations
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Dept. Molecular & Experimental, Scripps Research Institute, La Jolla, USA
Richard Milner
Bibliographic Information
Book Title: Astrocytes
Book Subtitle: Methods and Protocols
Editors: Richard Milner
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-452-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-451-3Published: 06 December 2011
Softcover ISBN: 978-1-4939-5859-7Published: 23 August 2016
eBook ISBN: 978-1-61779-452-0Published: 07 December 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 575
Topics: Neurosciences, Human Physiology