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Restriction Landmark Genome Scanning

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Epigenetics Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 791))

Abstract

Restriction landmark genome scanning (RLGS) method is a high-resolution two-dimensional electrophoresis system for analyses of the whole genome DNA which is including methylation status. It has been used for cloning genes of model animals and human genomes, detection of imprinted genes, and genome-wide methylation research in cancer. The conventional RLGS detected both polymorphism and methylated NotI sites between samples. Here, we have developed improved RLGS method with isoschizomer restriction enzymes such as MspI and HpaII to specifically detect methylated sites, using differential sensitivity of the restriction enzymes to methylated sequences. Recently, by using the genome database information, the RLGS spot sites were efficiently identified by this improved method. Then, genome methylation sites of Arabidopsis were mapped, and a unique inheritance was detected in methylated gene in rice. Now, epigenetic research becomes easy with the improved RLGS and it also can be applied for animal genome. Therefore, RLGS method is useful to explore for novel epigenetic phenomenon.

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Acknowledgments

We thank Paterson, A. H., Nishiguchi, M., Kakutani, T., Mizuno, Y., Yamashita, H., Nakamura, E., Asai, K., Higo, K., Hirochika H., Kawase, M., Tomioka, K., Habu, Y., Ohtake, Y., Ueda, T., Murakami, Y., Iijima, H., Takahashi, S., Nonaka, E., Nakamura, M., Hosobuchi, S., Noguchi, T., Saguchi, T., Fujita, T., and Seetharam, K., for their support.

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Authors and Affiliations

  1. Genetic Resources Center, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, Japan

    Hisato Okuizumi

  2. School of Pharmacy, Nihon University, Chiba, Japan

    Tomoko Takamiya

  3. Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan

    Yasushi Okazaki

  4. Omics Science Center, Riken, Yokohama, Kanagawa, Japan

    Yoshihide Hayashizaki

Authors
  1. Hisato Okuizumi
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  2. Tomoko Takamiya
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  3. Yasushi Okazaki
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  4. Yoshihide Hayashizaki
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Corresponding author

Correspondence to Hisato Okuizumi .

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Editors and Affiliations

  1. Dept. Biology, University of Alabama, Birmingham, University Blvd. 1300, Birmingham, 35294-1170, Alabama, USA

    Trygve O. Tollefsbol

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Okuizumi, H., Takamiya, T., Okazaki, Y., Hayashizaki, Y. (2011). Restriction Landmark Genome Scanning. In: Tollefsbol, T. (eds) Epigenetics Protocols. Methods in Molecular Biology, vol 791. Humana Press. https://doi.org/10.1007/978-1-61779-316-5_8

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  • DOI: https://doi.org/10.1007/978-1-61779-316-5_8

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