Abstract
Glia-neuron sandwich cocultures are “in vitro” cell systems suitable to evaluate cell-to-cell interactions relaying on the release of soluble factors, such as proinflammatory cytokines. This chapter presents a protocol to obtain a sandwich coculture from primary rat glial cells and hippocampal neurons. Furthermore, it provides details to evaluate the release of tumor necrosis factor-α by a biological assay and neuronal cell survival, as well as examples of practical application in the investigation of neuroinflammation and its impact on neuronal function.
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References
Allan, S. M., Rothwell, N. J. (2001) Cytokines and acute neurodegeneration. Nat Rev Neurosci. 10, 734–744.
Block M.L., Zecca L., Hong J.S. (2007) Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nat. Rev. Neurosci. 8, 57–69.
Viviani B., Corsini E., Galli C.L., Padovani A., Ciusani E., Marinovich M. (2000) Dying neural cells activate glia through the release of a protease product. Glia, 32, 84–90, 2000.
Biber K., Neumann H., Inoue K., Boddeke H.W.G.M. (2007) Neuronal “on” and “off” signals control microglia. TINS, 30, 596–602.
Villa P., Bigini P., Mennini T., Agnello D., Laragione T., Cagnotto A., Viviani B., Marinovich M., Cerami A., Coleman T.R., Brines M., Ghezzi P. (2003) Erythropoietin selectively attenuates cytokine production and inflammation in cerebral ischemia by targeting neuronal apoptosis. J. Exp. Med. 198, 971–975.
Vitkovic, L., Bockaert, J., and Jacque, C. (2000). “Inflammatory” cytokines: neuromodulators in normal brain? J Neurochem. 74, 457–71.
Viviani B., Gardoni F., Marinovich M. (2007). Cytokines and neuronal ion channels in health and disease. Int. Rev. Neurobiol. 82, 247–63.
Srinivasan, D., Yen, J. H., Joseph, D. J., and Friedman, W. (2004). Cell type-specific interleukin-1 beta signaling in the CNS. J Neurosci. 24, 6482–8.
Viviani B., Gardoni F., Bartesaghi S., Corsini E., Facchi, Galli CL, DiLuca M., Marinovich M. (2006) IL-1beta released by gp120 drives neural death through tyrosine phosphorylation and trafficking of NMDA receptors J. Biol. Chem.; 281, 30212–30222.
Viviani B., Corsini E., Galli C.L., Marinovich M. (1998) Glia increase degeneration of hippocampal neurons through release of tumor necrosis factor-a. Toxicol and Appl. Pharmacol., 150, 271–276.
Viviani B., Corsini E., Binaglia M., Galli C.L., Marinovich M. (2001) Reactive oxygen species generated by glia are responsible for neuron death induced by human immunodeficiency virus-glycoprotein 120 in vitro. Neuroscience. 107, 51–58.
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Boraso, M., Viviani, B. (2011). Glia-Neuron Sandwich Cocultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity. In: Costa, L., Giordano, G., Guizzetti, M. (eds) In Vitro Neurotoxicology. Methods in Molecular Biology, vol 758. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-170-3_9
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DOI: https://doi.org/10.1007/978-1-61779-170-3_9
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Publisher Name: Humana Press, Totowa, NJ
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