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Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS

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Laser Capture Microdissection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 755))

Abstract

The evolution of ovarian cancer will highly depend on platinum sensitivity or resistance. In an attempt to better understand this mechanism of resistance, we combined laser microdissection (LMD) of ovarian tumor cells with mass spectrometric techniques. To obtain disease-specific markers we isolated ovarian tumor cells with LMD without contamination of surrounding cells. Proteins were extracted by chemical lysis and subsequent peptide and protein information was gathered using surface-enhanced laser desorption/ionization-time of flight mass spectrometry.

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Authors and Affiliations

  1. Division of Gynecological Oncology, Department of Obstetrics and Gynecology, University Hospitals Leuven, Katholieke Universiteit Leuven, Leuven, Belgium

    Isabelle Cadron, Toon Van Gorp & Ignace Vergote

  2. Division of Gynaecological Oncology, Department of Obstetrics and Gynaecology, MUMC+, GROW – School for Oncology and Developmental Biology, Maastricht, The Netherlands

    Toon Van Gorp

  3. Department of Pathology, University Hospitals Leuven, Katholieke Universiteit Leuven, Leuven, Belgium

    Philippe Moerman

  4. Department of Molecular Cell Biology, University Hospitals Leuven, Katholieke Universiteit Leuven, Leuven, Belgium

    Etienne Waelkens

Authors
  1. Isabelle Cadron
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  2. Toon Van Gorp
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  3. Philippe Moerman
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  4. Etienne Waelkens
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  5. Ignace Vergote
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Corresponding author

Correspondence to Isabelle Cadron .

Editor information

Editors and Affiliations

  1. , Department of Pathology, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Graeme I. Murray

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Cadron, I., Van Gorp, T., Moerman, P., Waelkens, E., Vergote, I. (2011). Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS. In: Murray, G. (eds) Laser Capture Microdissection. Methods in Molecular Biology, vol 755. Humana Press. https://doi.org/10.1007/978-1-61779-163-5_12

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  • DOI: https://doi.org/10.1007/978-1-61779-163-5_12

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-162-8

  • Online ISBN: 978-1-61779-163-5

  • eBook Packages: Springer Protocols

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