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Generation of Quorum Quenching Antibodies

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Quorum Sensing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 692))

Abstract

The exchange of information within and among bacterial populations using small diffusible molecules has been termed “quorum sensing” (QS). Due to the extracellular distribution of the QS autoinducer molecules and the evolutionary highly conserved nature of signaling components, microbial QS systems represent an excellent target for anti-infective immunotherapy. Recently, we have described the generation of quorum quenching monoclonal antibodies (mAbs) against acyl homoserine lactones (AHL) used by Pseudomonas aeruginosa as well as Staphylococcal autoinducing peptides (AIP). These mAbs suppressed QS signaling in bacteria and neutralized AHL-mediated cytotoxic effects in vitro, as well as protected animals in Staphylococcus aureus infection models.

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Acknowledgments

This work was supported by the National Institutes of Health (AI080715 to G.F.K). This is manuscript number 20549 from The Scripps Research Institute.

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Authors and Affiliations

  1. Departments of Chemistry and Immunology & Microbial Science, The Scripps Research Institute, La Jolla, CA, USA

    Gunnar F. Kaufmann, Junguk Park & Kim D. Janda

  2. The Skaggs Institute for Chemical Biology and Departments of Chemistry and Immunology & Microbial Science, The Scripps Research Institute, La Jolla, CA, USA

    Alexander V. Mayorov

  3. Antibody Production Core Facility, The Scripps Research Institute, La Jolla, CA, USA

    Diane M. Kubitz

  4. Worm Institute for Research and Medicine (WIRM), The Skaggs Institute for Chemical Biology, La Jolla, CA, USA

    Kim D. Janda

Authors
  1. Gunnar F. Kaufmann
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  2. Junguk Park
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  3. Alexander V. Mayorov
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  4. Diane M. Kubitz
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  5. Kim D. Janda
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Corresponding author

Correspondence to Gunnar F. Kaufmann .

Editor information

Editors and Affiliations

  1. Health Sciences Center, Department of Surgery, Texas Tech University, 4th Street 3601, Lubbock, 79430, Texas, USA

    Kendra P. Rumbaugh

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Kaufmann, G.F., Park, J., Mayorov, A.V., Kubitz, D.M., Janda, K.D. (2011). Generation of Quorum Quenching Antibodies. In: Rumbaugh, K. (eds) Quorum Sensing. Methods in Molecular Biology, vol 692. Humana Press. https://doi.org/10.1007/978-1-60761-971-0_22

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  • DOI: https://doi.org/10.1007/978-1-60761-971-0_22

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-970-3

  • Online ISBN: 978-1-60761-971-0

  • eBook Packages: Springer Protocols

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