Abstract
The ability to amplify genetic material using PCR has transformed the field of diagnostics. Now any organism can be detected by identifying the presence of specific nucleic acids. However, there still remain areas in which traditional PCR cannot easily be applied. In this chapter, we describe a different flavour of PCR in which an enzyme acts on a DNA substrate to convert it into a DNA product that can be detected by PCR. This opens up new areas for the application of the PCR technique and we will show how the technique can be used to detect immunoconjugates, bacterial drug resistance, and bacterial contamination.
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Innis, M.A., Gelfand, D.H., Sninsky, J.J, and White, T.J. (Eds.) (1990) PCR Protocols. A guide to methods and application. Academic Press Inc, San Diego, CA.
Adler, M., Wacker, R., and Niemeyer, C.M. (2008) Sensitivity by combination: immuno-PCR and related technologies. Analyst 133, 702–18.
Eder, A.F., Kennedy, J.M., Dy, B.A., Notari, E.P., Weiss, J.W., Fang, C.T., Wagner, S., Dodd, R.Y., and Benjamin, R.J. (2007) Bacterial screening of apheresis platelets and the residual risk of septic transfusion reactions: the American Red Cross experience (2004–2006). Transfusion 47, 1134–42.
Dierkes, C., Ehrenstein, B., Siebig, S., Linde, H.J., Reischl, U., and Salzberger, B. (2009) Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect. Dis. 11, 126.
De Luca, M. and McElroy, W.D. (1978) Purification and properties of firefly luciferase. Meth. Enzymol. 57, 3–15.
Kricka, L.J. (1988) Clinical and biochemical applications of luciferases and luciferins. Anal. Biochem. 175, 14–21.
Lundin, A., Hasenson, M., Persson, J., and Pousette, A. (1986) Estimation of biomass in growing cell lines by adenosine triphosphate assay. Meth. Enzymol. 133, 27–42.
Stanley, P.E. (1992) A survey of more than 90 commercially available luminometers and imaging devices for low-light measurements of chemiluminescence and bioluminescence, including instruments for manual, automatic and specialized operation, for HPLC, LC, GLC and microtitre plates. Part 1: descriptions. J. Biolumin. Chemilumin. 7, 77–108.
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© 2011 Humana Press
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Wilson, S.M. (2011). Novel Applications of PCR Through the Use of DNA Substrates. In: Park, D. (eds) PCR Protocols. Methods in Molecular Biology, vol 687. Humana Press. https://doi.org/10.1007/978-1-60761-944-4_17
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DOI: https://doi.org/10.1007/978-1-60761-944-4_17
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