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Vaccine Adjuvants pp 261–285Cite as

Determining the Activity of Mucosal Adjuvants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 626))

Abstract

Mucosal vaccination offers the advantage of blocking pathogens at the portal of entry, improving patient’s compliance, facilitating vaccine delivery, and decreasing the risk of unwanted spread of infectious agents via contaminated syringes.

Recent advances in vaccinology have created an array of vaccine constructs that can be delivered to mucosal surfaces of the respiratory, gastrointestinal, and genitourinary tracts using intranasal, oral, and vaginal routes. Due to the different characteristics of mucosal immune response, as compared with systemic response, mucosal immunization requires particular methods of antigen presentation. Well-tolerated adjuvants that enhance the efficacy of such vaccines will play an important role in mucosal immunization. Among promising mucosal adjuvants, mutants of cholera toxin and the closely related heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli present powerful tools, augmenting the local and systemic serum antibody response to co-administered antigens.

In this chapter, we describe the formulation and application of vaccines using the genetically modified LTK63 mutant as a prototype of the family of these mucosal adjuvants and the tools to determine its activity in the mouse model.

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Acknowledgment

We are grateful to Markus Hilleringmann for critical reading of the manuscript and Derek O’Hagan for general support.

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Authors and Affiliations

  1. Novartis Vaccines and Diagnostics, Siena, Italy

    Barbara C. Baudner & Giuseppe Del Giudice

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  1. Barbara C. Baudner
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  2. Giuseppe Del Giudice
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Correspondence to Barbara C. Baudner .

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  1. Dept. Cardio & Vascular Sciences, St. George's University of London, Cranmer Terrace, London, SW17 0RE, United Kingdom

    Gwyn Davies

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Baudner, B.C., Giudice, G.D. (2010). Determining the Activity of Mucosal Adjuvants. In: Davies, G. (eds) Vaccine Adjuvants. Methods in Molecular Biology, vol 626. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-585-9_18

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  • DOI: https://doi.org/10.1007/978-1-60761-585-9_18

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