Abstract
Oxycodone is a semisynthetic opioid analgesic used in pain management. In the following method, gas chromatography-mass spectrometry (GC-MS) is used to determine the presence and concentration of the drug in blood and urine. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted using mixed-mode solid phase extraction and derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) producing trimethylsilyl derivatives. The final extracts are then analyzed using selected ion monitoring GC-MS.
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References
Stout, P.R. and Farrell, L.J. (2003) Opioids: Effects on human performance and behavior. Forensic Sci Rev, 15, 29–59. Review.
Gutstein, H.B. and Akil, H. (2006) Opioid Analgesics. In Brunton, L.L., Lazo, J.S. and Parker, K.L. (eds), Goodman & Gilman's the Pharmacological Basis of Therapeutics, 11th edition. McGraw-Hill, New York, pp. 547–590.
Lewis, L.S. (2006) Opioids. In Flomenbaum, N.E., Goldfrank, L.R., Hoffman, R.S., Howland, M.A., Lewin, N.A. and Nelson, L.S. (eds), Goldfrank's Toxicologic Emergencies, 8th edition. McGraw-Hill, New York, pp. 590–613.
Clean Screen Extraction Column Application Manual, United Chemical Technologies (2009).
Ropero-Miller, J.D., Lambing, M.K. and Winecker, R.E. (2002) Simultaneous quantitation of opioids in blood by GC-EI-MS analysis following deproteination, detautomerization of keto analytes, solid-phase extraction, and trimethylsilyl derivatization. J Anal Toxicol, 26, 524–528.
Moore, K.A., Ramcharitar, V., Levine, B. and Fowler, D. (2003) Tentative identification of novel oxycodone metabolites in human urine. J Anal Toxicol, 27, 346–352.
Meatherall, R. (2005) GC-MS quantitation of codeine, morphine, 6-acetylmorphine, hydrocodone, hydromorphone, oxycodone, and oxymorphone in blood. J Anal Toxicol, 29, 301–308.
Moore, C., Rana, S. and Coulter, C. (2007) Determination of meperidine, tramadol and oxycodone in human oral fluid using solid phase extraction and gas chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci, 850, 370–375.
McKinley, S., Snyder, J.J., Welsh, E., Kazarian, C.M., Jamerson, M.H. and Klette, K.L. (2007) Rapid quantification of urinary oxycodone and oxymorphone using fast gas chromatography-mass spectrometry. J Anal Toxicol, 31, 434–441.
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Goldberger, B.A., Chronister, C.W., Merves, M.L. (2010). Quantitation of Oxycodone in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS). In: Garg, U., Hammett-Stabler, C. (eds) Clinical Applications of Mass Spectrometry. Methods in Molecular Biology, vol 603. Humana Press. https://doi.org/10.1007/978-1-60761-459-3_44
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DOI: https://doi.org/10.1007/978-1-60761-459-3_44
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