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MR Tracking of Stem Cells in Living Recipients

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Neural Cell Transplantation

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 549))

Summary

Noninvasive cellular imaging allows the real-time tracking of grafted cells as well as the monitoring of their migration. Several techniques for in vivo cellular imaging are available that permit the characterization of transplanted cells in a living organism, including magnetic resonance imaging (MRI), bioluminescence, positron emission tomography, and multiple photon microscopy. All of these methods, based on different principles, provide distinctive, usually complementary information. In this review, we will focus on cell tracking using MRI, since MRI is noninvasive, clinically transferable, and displays good resolution, ranging from 50μm in animal experiments up to 300μm using whole body clinical scanners. In addition to information about grafted cells, MRI provides information about the surrounding tissue (i.e., lesion size, edema, inflammation), which may negatively affect graft survival or the functional recovery of the tissue. Transplanted cells are labeled with MR contrast agents in vitro prior to transplantation in order to visualize them in the host tissue. The chapter will focus on the use of superparamagnetic iron oxide nanoparticles (SPIO), because they have strong effects on T2 relaxation yet do not affect cell viability, and will provide an overview of different modifications of SPIO and their use in MR tracking in living organisms.

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Acknowledgments

This work was supported by grants from the Academy of Sciences of the Czech Republic AV0Z50390703, KAN201110651, the Ministry of Education, Youth and Sports of the Czech Republic 1M0021620803, LC554, the National Grant Agency of the Czech Republic GACR 309/06/1594, the grant agency of the Ministry of Health NR8339-3, and the EC – FP6 project DiMI: LSHB-CT-2005–512146.

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Authors and Affiliations

  1. Institute of Experimental Medicine ASCR, Víde ská, 1083, 140 20 Prague 4, Czech Republic

    Eva Syková & Pavla Jendelová

  2. Department of Radiodiagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic

    Vít Herynek

Authors
  1. Eva Syková
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  2. Pavla Jendelová
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  3. Vít Herynek
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Correspondence to Eva Syková .

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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Syková, E., Jendelová, P., Herynek, V. (2009). MR Tracking of Stem Cells in Living Recipients. In: Gordon, D., Scolding, N. (eds) Neural Cell Transplantation. Methods in Molecular Biology™, vol 549. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-931-4_14

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    • DOI: https://doi.org/10.1007/978-1-60327-931-4_14

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    • Publisher Name: Humana Press, Totowa, NJ

    • Print ISBN: 978-1-60327-930-7

    • Online ISBN: 978-1-60327-931-4

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