Abstract
RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos.
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Acknowledgements
This research was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.
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Biris, K.K., Yamaguchi, T.P. (2014). Two-Color In Situ Hybridization of Whole-Mount Mouse Embryos. In: Lewandoski, M. (eds) Mouse Molecular Embryology. Methods in Molecular Biology, vol 1092. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-60327-292-6_2
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DOI: https://doi.org/10.1007/978-1-60327-292-6_2
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Publisher Name: Humana Press, Boston, MA
Print ISBN: 978-1-60327-290-2
Online ISBN: 978-1-60327-292-6
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