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Deriving Mouse Spermatogonial Stem Cell Lines

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Germline Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 450))

Summary

In adult males, spermatogonial stem cells function to replenish developing gametes that are continuously released from the testes as mature spermatozoa. Because of their potential importance to research, medicine, industry, and conservation, numerous attempts have been made in the past to cultivate sperma-togonial stem cells in vitro. However, only recently have culture methods been established that effectively promote the proliferation of mammalian spermatogonial stem cells in vitro. We describe a simple and reproducible protocol for the derivation and maintenance of mouse spermatogonial stem cell lines that proliferate for long periods of time in culture.

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References

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Author information

Authors and Affiliations

  1. Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Ilaria Falciatori PhD

  2. Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Kate Lillard-Wetherell PhD

  3. Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Zhuoru Wu PhD

  4. Department of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA

    F. Kent Hamra PhD

  5. Department of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA

    David L. Garbers PhD

Authors
  1. Ilaria Falciatori PhD
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  2. Kate Lillard-Wetherell PhD
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  3. Zhuoru Wu PhD
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  4. F. Kent Hamra PhD
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  5. David L. Garbers PhD
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Editor information

Editors and Affiliations

  1. National Cancer Institute, Frederick, MD, USA

    Steven X. Hou  & Shree Ram Singh  & 

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© 2008 Humana Press, a part of Springer Science + Business Media, LLC

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Falciatori, I., Lillard-Wetherell, K., Wu, Z., Hamra, F.K., Garbers, D.L. (2008). Deriving Mouse Spermatogonial Stem Cell Lines. In: Hou, S.X., Singh, S.R. (eds) Germline Stem Cells. Methods in Molecular Biology™, vol 450. Humana Press. https://doi.org/10.1007/978-1-60327-214-8_13

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  • DOI: https://doi.org/10.1007/978-1-60327-214-8_13

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-213-1

  • Online ISBN: 978-1-60327-214-8

  • eBook Packages: Springer Protocols

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