Summary
Staphylococcus aureus is a leading cause of human infections worldwide and causes a variety of diseases ranging in severity from mild to life-threatening. The ability of S. aureus to cause disease is based in part on its ability to subvert the innate immune system. Advances in genome-wide analysis of host–pathogen interactions have provided the necessary tools to investigate molecular factors that directly contribute to S. aureus pathogenesis. This chapter describes methods to analyze gene expression in S. aureus during interaction with human neutrophils.
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Acknowledgments
This work was supported by the NIH-NRRI grant P20RR020185 (J.M.V.) and the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health (F.R.D.).
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Voyich, J.M., Sturdevant, D.E., DeLeo, F.R. (2008). Analysis of Staphylococcus aureus Gene Expression During PMN Phagocytosis . In: DeLeo, F.R., Otto, M. (eds) Bacterial Pathogenesis. Methods in Molecular Biology™, vol 431. Humana Press. https://doi.org/10.1007/978-1-60327-032-8_9
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DOI: https://doi.org/10.1007/978-1-60327-032-8_9
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