Abstract
Animal cloning is becoming increasingly useful for its applications in biological inquiry and for its potential use in pharmaceutical, medical, and agricultural fields. Due to the complexity of the numerous steps required in reconstructing oocytes by nuclear transfer, detailed protocols are required to minimize the developmental damages inflicted during these manipulations and to standardize procedures across laboratories. Moreover, because oogenesis and early embryogenesis differ widely among mammalian species, it is essential that protocols be adapted according to each species concerned. Our objective here is to detail the protocols that have been most successful in producing laboratory and domestic animal clones.
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References
Briggs, R. and King, T.J. (1952) Nuclear transplantation studies on the early gastrula (Rana pipiens). Dev. Biol. 2, 252–270.
Illmensee, K. and Hoppe, P.C. (1981) Nuclear transplantation in Mus musculus: developmental potential of nuclei from preimplantation embryos. Cell 23, 9–1.
Willadsen, S.M. (1986) Nuclear transplantation in sheep embryos. Nature 320, 63–65.
Smith, L.C. and Wilmut, I. (1989) Influence of nuclear and cytoplasmic activity on the development in vivo of sheep embryos after nuclear transplantation. Biol. Reprod. 40, 1027–1035.
Sims, M. and First, N.L. (1993) Production of calves by transfer of nuclei from cultured inner cell mass cells. Proc. Natl. Acad. Sci. USA 91, 6143–6147.
Campbell, K.H., McWhir, J., Ritchie, W.A. and Wilmut, I. (1996) Sheep cloned by nuclear transfer from a cultured cell line. Nature 380, 64–66.
Wilmut, I., Schnieke, A.E., McWhir, J., Kind, A.J. and Campbell, K.H. (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810–813.
Verma, P.J. and Trounson, A.O. (2006) Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis. Humana Press, Boston.
Rosenkrans, C.F. and First, N.L. (1994) Effects of free amino acids and vitamins on cleavage and development rate of bovine zygotes in vitro. J. Anim. Sci 72, 434–437.
Petters, R.M. and Wells, K. D. (1993) Culture of pig embryos. J. Reprod. Fertil. Suppl. 48, 61–73.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Smith, L.C., Yoo, JG. (2009). Animal Cloning by Somatic Cell Nuclear Transfer. In: Vaillancourt, C., Lafond, J. (eds) Human Embryogenesis. Methods in Molecular Biology, vol 550. Humana Press. https://doi.org/10.1007/978-1-60327-009-0_17
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DOI: https://doi.org/10.1007/978-1-60327-009-0_17
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