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Comparative Genomic Hybridization: Microarray Design and Data Interpretation

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DNA Microarrays for Biomedical Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 529))

Abstract

Microarray-based Comparative Genomic Hybridization (array-CGH) has been applied for a decade to screen for submicroscopic DNA gains and losses in tumor and constitutional DNA samples. This method has become increasingly flexible with the integration of new biological resources generated by genome sequencing projects. In this chapter, we describe alternative strategies for whole genome screening and high resolution breakpoint mapping of copy number changes by array-CGH, as well as tools available for accurate analysis of array-CGH experiments. Although most methods listed here have been designed for microarrays comprising large-insert clones, they can be adapted easily to other types of microarray platforms, such as those constructed from printed or synthesized oligonucleotides.

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Acknowledgment

This work was supported by the Wellcome Trust.

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  1. Wellcome Trust, Sanger Institute, Cambridge, UK

    Richard Redon & Nigel P. Carter

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  1. Richard Redon
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  2. Nigel P. Carter
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Redon, R., Carter, N.P. (2009). Comparative Genomic Hybridization: Microarray Design and Data Interpretation. In: Dufva, M. (eds) DNA Microarrays for Biomedical Research. Methods in Molecular Biology, vol 529. Humana Press. https://doi.org/10.1007/978-1-59745-538-1_3

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    • DOI: https://doi.org/10.1007/978-1-59745-538-1_3

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-934115-69-5

    • Online ISBN: 978-1-59745-538-1

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