Abstract
The authors solved a microscopic model that describes mixtures of a saturated phospholipid, an unsaturated phospholipid, and cholesterol. The method employed was the self-consistent field approximation. The model was capable of producing several classes of phase diagram, but only one of them showed a liquid-liquid coexistence region. The phospholipids in the cholesterol-rich liquid are more ordered than those in the cholesterol-poor liquid. Within this model, coexistence of two liquids in the ternary system is intimately tied to such coexistence in the binary cholesterol-saturated phospholipid system.
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References
Komura, S., Shirotori, H., Olmsted, P. D., and Andelman, D. (2004) Lateral phase separation in mixtures of lipids and cholesterol. Europhys. Lett. 67, 321–327.
Radhakrishnan, A. and McConnell, H. (2005) Condensed complexes in vesicles containing cholesterol and phospholipids. Proc. Nat. Acad. Sci. 102, 12,662–12,666.
Ipsen, J. H., Karlstrom, G., Mouritsen, O. G., Wennerstrom, H., and Zuckermann, M. J. (1987) Phase equilibria in the phosphatidylcholine-cholesterol system. Biochim. Biophys. Acta 905, 162–172.
Nielsen, M., Miao, L., Ipsen, J. H., Zuckermann, M. J., and Mouritsen, O. G. (1999) Off-lattice model for the phase behavior of lipid-cholesterol bilayers. Phys. Rev. E 59, 5790–5803.
Pandit, S., Jakobsson, E., and Scott, H. (2004) Simulation of the early stages of nano-domain formation in mixed bilayers of sphingomyelin, cholesterol and dioleylphosphatidylycholine. Biophys. J. 87, 3312–3322.
Elliott, R., Szleifer, I., and Schick, M. (2006) Phase diagram of a ternary mixture of cholesterol and saturated and unsaturated lipids calculated from a microscopic model. Phys. Rev. Lett. 96, 098101.
Vist, M. and Davis, J. (1990) Phase equilibria of cholesterol/dipalmitoylphosphatidylcholine mixtures:nuclear magnetic resonance and differential scanning calorimetry. Biochemistry 29, 451–464.
de Almeida, R., Fedorov, A., and Prieto, M. (2003) Sphingomyelin/phosphatidylcholine/cholesterol phase diagram: boundaries and composition of lipid rafts. Biophys. J. 85, 2406–2416.
Pokorny, A., Yandek, L., Elegbede, A., Hinderliter, A., and Almeida, P. (2006) Temperature and composition dependence of the interaction of d-lysin with ternary mixtures of sphingomyelin/cholesterol/POPC. Biophys. J. 91, 2184–2197.
Veatch, S. and Keller, S. (2005) Miscibility phase diagrams of giant vesicles containing sphingomyelin. Phys. Rev. Lett. 94, 148101.
Ben-Shaul, A., Szleifer, I., and Gelbart, W. (1985) Chain organization and thermodynamics in micelles and bilayers. I. Theory. J. Chem. Phys. 83, 3597–3611.
Elliott, R., Katsov, K., Schick, M., and Szleifer, I. (2005) Phase separation of saturated and mono-unsaturated lipids as determined from a microscopic model. J. Chem. Phys. 122, 144904.
Fattal, D. R. and Ben-Shaul, A. (1994) Mean-field calculations of chain packing and conformational statistics in lipid bilayers: Comparison with experiments and molecular dynamics studies. Biophys. J. 67, 983–995.
Szleifer, I., Ben-Shaul, A., and Gelbart, W. M. (1986) Chain statistics in micelles and bilayers: effects of surface roughness and internal energy. J. Chem. Phys. 85, 5345–5358.
Flory, P. J. (1969) Statistical Mechanics of Chain Molecules. Wiley-Interscience, New York.
Finegold, L. and Singer, M. (1986) The metastability of saturated phosphatidylcholines depends on the acyl chain length. Biochim. Biophys. Acta 855, 417–420.
Nagle, J. F. and Tristam-Nagle, S. (2000) Structure of lipid bilayers. Biochim. Biophys. Acta 1469, 159–195.
Khelashvili, G., Pandit, S., and Scott, H. (2005) Self-consistent mean-field model based on molecular dynamics: Application to lipid-cholesterol bilayers. J. Chem. Phys. 123, 034910.
Pandit, S., Khelashvili, G., Jakobsson, E., Grama, A., and Scott, H. (2006) Lateral organization in lipid-cholesterol mixed bilayers. Biophys. J. 92, 440–447.
Veatch, S., Gawrisch, K., and Keller, S. (2006) Closed-loop miscibility gap and quantitative tie-lines in ternary membranes containing diphytanol PC. Biophys. J. 90, 4428–4436.
Swamy, M., Ciani, L., Ge, M, et al. (2006) Coexisting domains in the plasma membranes of live cells characterized by spin label esr spectroscopy. Biophys. J. 90, 4466–4478.
Veatch, S. L., Polozov, I., Gawrisch, K., and Keller, S. (2004) Liquid domains in vesicles investigated by nmr and fluorescence microscopy. Biophys. J. 86, 2910–2922.
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Elliott, R., Szleifer, I., Schick, M. (2007). A Microscopic Model Calculation of the Phase Diagram of Ternary Mixtures of Cholesterol and Saturated and Unsaturated Phospholipids. In: McIntosh, T.J. (eds) Lipid Rafts. Methods in Molecular Biology, vol 398. Humana Press. https://doi.org/10.1007/978-1-59745-513-8_20
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DOI: https://doi.org/10.1007/978-1-59745-513-8_20
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