Summary
Characterization of gene expression programs and pathways important for normal and cancer stem cells has become an active area of investigation. Microarray analysis of various cell populations provides an opportunity to assess genomewide expression programs to define cellular identity and to potentially identify pathways activated in various stem cells. Here we describe methods to isolate a leukemia stem cell population, amplify RNA, and perform microarray analyses.
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Acknowledgments
We thank all the members of the Armstrong lab for their help in developing these protocols. Also, we would like to thank members of the Broad Institute cancer program and Todd R. Golub for help in developing these approaches and for ongoing collaborative efforts.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Krivtsov, A.V., Wang, Y., Feng, Z., Armstrong, S.A. (2009). Gene Expression Profiling of Leukemia Stem Cells. In: Eric So, C.W. (eds) Leukemia. Methods in Molecular Biology™, vol 538. Humana Press. https://doi.org/10.1007/978-1-59745-418-6_11
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DOI: https://doi.org/10.1007/978-1-59745-418-6_11
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