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Functional Proteomics pp 79–93Cite as

Flow Cytometric Analysis of Cell Membrane Microparticles

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 484))

Abstract

Cell membrane microparticles (MPs) are phospholipid microvesicles shed from the plasma membrane of most eukaryotic cells undergoing activation or apoptosis. The presence of MPs is common in healthy individuals. However, an increase in their release is a controlled event and is considered a hallmark of cellular alteration. Microparticles display cell surface proteins that indicate their cellular origin. In addition, they may also express other markers, e.g., markers of cellular activation. Elevated levels of circulating MPs are associated with various vascular pathologies and their pathogenic potential has been widely documented. MPs have been analyzed in plasma and cell cultures by means of flow cytometry or solid phase assays. Here we present a three-color flow cytometric assay for immunophenotyping of MPs in plasma. This assay has been used to study elevated counts of different phenotypes of circulating endothelial MPs in several hematological and vascular diseases. A modified version of this assay can also be, used for MP analysis in blood products and cell cultures.

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Author information

Authors and Affiliations

  1. Laboratory of Cellular Hematology, CBER FDA, Rockville, MD, USA

    Monique P. Gelderman & Jan Simak

Authors
  1. Monique P. Gelderman
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  2. Jan Simak
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Editor information

Editors and Affiliations

  1. Laboratoire de Bioinformatique et Génomique Intégratives, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France

    Julie D. Thompson

  2. Department of Protein Science Helmholtz Zentrum München, German Research Center for Environmental Health, Munich-Neuherberg, Germany

    Marius Ueffing

  3. LSMBO, ECPM, Institut Pluridisciplinaire Hubert Curien, Strasbourg, France

    Christine Schaeffer-Reiss

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© 2008 Humana Press, Totowa, NJ

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Gelderman, M.P., Simak, J. (2008). Flow Cytometric Analysis of Cell Membrane Microparticles. In: Thompson, J.D., Ueffing, M., Schaeffer-Reiss, C. (eds) Functional Proteomics. Methods in Molecular Biology, vol 484. Humana Press. https://doi.org/10.1007/978-1-59745-398-1_6

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  • DOI: https://doi.org/10.1007/978-1-59745-398-1_6

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-971-0

  • Online ISBN: 978-1-59745-398-1

  • eBook Packages: Springer Protocols

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