Skip to main content

Specimen Collection and Handling

Standardization of Blood Sample Collection

  • Protocol
Clinical Proteomics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 428))

Summary

Preanalytical variables can alter the analysis of blood-derived samples. Prior to the analysis of a blood sample, multiple steps are necessary to generate the desired specimen. The choice of blood specimens, its collection, handling, processing, and storage are important aspects since these characteristics can have a tremendous impact on the results of the analysis.

The awareness of clinical practices in medical laboratories and the current knowledge allow for identification of specific variables that affect the results of a proteomic study. The knowledge of preanalytical variables is a prerequisite to understand and control their impact.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Vitzthum F, Behrens F, Anderson NL, Shaw JH. (2005) Proteomics: from basic research to diagnostic application. A review of requirements and needs. J. Proteome Res. 4, 1086–97.

    Article  PubMed  CAS  Google Scholar 

  2. Lathrop JT, Anderson NL, Anderson NG, Hammond DJ. (2003) Therapeutic potential of the plasma proteome. Curr. Opin. Mol. Ther. 5, 250–7.

    PubMed  CAS  Google Scholar 

  3. Wang W, Zhou H, Lin H, Roy S, Shaler TA, Hill LR et al. (2003) Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards. Anal. Chem. 75, 4818–26.

    Article  PubMed  CAS  Google Scholar 

  4. Anderson NL, Anderson NG. (2002) The human plasma proteome: history, character, and diagnostic prospects. Mol. Cell. Proteomics 1, 845–67.

    Article  PubMed  CAS  Google Scholar 

  5. Omenn GS. (2004) The Human Proteome Organization Plasma Proteome Project pilot phase: reference specimens, technology platform comparisons, and standardized data submissions and analyses. Proteomics 4, 1235–40.

    Article  PubMed  CAS  Google Scholar 

  6. Plebani M, Carraro P. (1997) Mistakes in a stat laboratory: types and frequency. Clin. Chem. 43, 1348–51.

    PubMed  CAS  Google Scholar 

  7. Burtis CA, Ashwood E. (eds) (2001) Fundamentals of Clinical Chemistry. Saunders, Philadelphia.

    Google Scholar 

  8. Guder WG, Narayanan S, Wisser H, Zawata B. (2003) Samples: From the Patient to the Laboratory. The Impact of Preanalytical Variables on the Quality of Laboratory Results. GIT Verlag, Darmstadt, Germany.

    Google Scholar 

  9. Evans MJ, Livesey JH, Ellis MJ, Yandle TG. (2001) Effect of anticoagulants and storage temperatures on stability of plasma and serum hormones. Clin. Biochem 34, 107–12.

    Article  PubMed  CAS  Google Scholar 

  10. Omenn GS, States DJ, Adamski M, Blackwell TW, Menon R, Hermjakob H et al. (2005) Overview of the HUPO Plasma Proteome Project: results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database. Proteomics 5, 3226–45.

    Article  PubMed  CAS  Google Scholar 

  11. Rai AJ, Gelfand CA, Haywood BC, Warunek DJ, Yi J, Schuchard MD et al. (2005) HUPO Plasma Proteome Project specimen collection and handling: towards the standardization of parameters for plasma proteome samples. Proteomics 5, 3262–77.

    Article  PubMed  CAS  Google Scholar 

  12. Villanueva J, Shaffer DR, Philip J, Chaparro CA, Erdjument-Bromage H, Olshen AB et al. (2006) Differential exoprotease activities confer tumor-specific serum peptidome patterns. J. Clin. Invest . 116, 271–84.

    Article  PubMed  CAS  Google Scholar 

  13. Liotta LA, Petricoin EF. (2006) Serum peptidome for cancer detection: spinning biologic trash into diagnostic gold. J. Clin. Invest. 116, 26–30.

    Article  PubMed  CAS  Google Scholar 

  14. Tammen H, Schulte I, Hess R, Menzel C, Kellmann M, Schulz-Knappe P. (2005) Prerequisites for peptidomic analysis of blood samples: I. Evaluation of blood specimen qualities and determination of technical performance characteristics. Comb. Chem. High Throughput Screen. 8, 725–33.

    Article  PubMed  CAS  Google Scholar 

  15. Holland NT, Smith MT, Eskenazi B, Bastaki M. (2003) Biological sample collection and processing for molecular epidemiological studies. Mutat. Res. 543, 217–34.

    Article  PubMed  CAS  Google Scholar 

  16. Landi MT, Caporaso N. (1997) Sample collection, processing and storage. IARC Sci. Publ. 223–36.

    Google Scholar 

  17. Tammen H, Schulte I, Hess R, Menzel C, Kellmann M, Mohring T, Schulz-Knappe P. (2005) Peptidomic analysis of human blood specimens: comparison between plasma specimens and serum by differential peptide display. Proteomics 13, 3414–22.

    Article  Google Scholar 

  18. Favaloro EJ, Soltani S, McDonald J. (2004) Potential laboratory misdiagnosis of hemophilia and von Willebrand disorder owing to cold activation of blood samples for testing. Am. J. Clin. Pathol. 122, 686–92.

    Article  PubMed  CAS  Google Scholar 

  19. Mustard JF, Kinlough-Rathbone RL, Packham MA. (1989) Isolation of human platelets from plasma by centrifugation and washing. Methods Enzymol. 169, 3–11.

    Article  PubMed  CAS  Google Scholar 

  20. Schuchard MD, Mehigh RJ, Cockrill SL, Lipscomb GT, Stephan JD, Wildsmith J et al. (2005) Artifactual isoform profile modification following treatment of human plasma or serum with protease inhibitor, monitored by 2-dimensional electrophoresis and mass spectrometry. Biotechniques 39, 239–47.

    Article  PubMed  CAS  Google Scholar 

  21. Jeffrey DH, Deidra B, Keith H, Shu-Pang H, Deborah LR, Gregory JO, Stanley AH. (2004) An Investigation of Plasma Collection, Stabilization, and Storage Procedures for Proteomic Analysis of Clinical Samples. Humana, Totowa, NJ.

    Google Scholar 

  22. Rai AJ, Vitzthum F. (2006) Effects of preanalytical variables on peptide and protein measurements in human serum and plasma: implications for clinical proteomics. Expert Rev. Proteomics 3, 409–26.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Tammen, H. (2008). Specimen Collection and Handling. In: Vlahou, A. (eds) Clinical Proteomics. Methods in Molecular Biology™, vol 428. Humana Press. https://doi.org/10.1007/978-1-59745-117-8_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-59745-117-8_2

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-837-9

  • Online ISBN: 978-1-59745-117-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics