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Bioluminescent Imaging pp 107–126Cite as

Multiplex Imaging of Polymicrobial Communities—Murine Models to Study Oral Microbiome Interactions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2081))

Abstract

Similar to other mucosal surfaces of the body, the oral cavity hosts a diverse microbial flora that live in polymicrobial biofilm communities. It is the ecology of these communities that are the primary determinants of oral health (symbiosis) or disease (dysbiosis). As such, both symbiosis and dysbiosis are inherently polymicrobial phenomena. In an effort to facilitate studies of polymicrobial communities within rodent models, we developed a suite of synthetic luciferases suitable for multiplexed in situ analyses of microbial ecology and specific gene expression. Using this approach, it is feasible to noninvasively measure multiple luciferase signals in vivo with both spatial and temporal resolution. In the following chapter, we describe the relevant details and protocols used to establish a biophotonic imaging platform for the study of experimental polymicrobial oral biofilms and abscesses in mice. The protocols described here are specifically tailored for use with oral streptococci, but the general strategies are adaptable for a wide range of polymicrobial infection studies using other species.

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Acknowledgments

This work was supported by an NIH/NIDCR grant R35DE0282252 to J.M. and NIH/NIDCR grants R01DE021726 and R56DE021726 to J.K.

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Authors and Affiliations

  1. Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR, USA

    Jens Kreth, Yasser M. Abdelrahman & Justin Merritt

  2. Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt

    Yasser M. Abdelrahman

  3. Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR, USA

    Justin Merritt

Authors
  1. Jens Kreth
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  2. Yasser M. Abdelrahman
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  3. Justin Merritt
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Corresponding author

Correspondence to Justin Merritt .

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Editors and Affiliations

  1. The Center for Environmental Biotechnology, The University of Tennessee, 490 BioTech Inc., Knoxville, TN, USA

    Steven Ripp

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Kreth, J., Abdelrahman, Y.M., Merritt, J. (2020). Multiplex Imaging of Polymicrobial Communities—Murine Models to Study Oral Microbiome Interactions. In: Ripp, S. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 2081. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9940-8_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9940-8_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9939-2

  • Online ISBN: 978-1-4939-9940-8

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