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Genotype Phenotype Coupling pp 19–41Cite as

Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2070))

Abstract

Engineered protein scaffolds have made a tremendous contribution to the panel of affinity tools owing to their favorable biophysical properties that make them useful for many applications. In 2007, our group paved the way for using archaeal Sul7d proteins for the design of artificial affinity ligands, so-called Affitins. For many years, Sac7d and Sso7d have been used as molecular basis to obtain binders for various targets. Recently, we characterized their old gifted protein family and identified Aho7c, originating from Acidianus hospitalis, as the shortest member (60 amino-acids) with impressive stability (96.5 °C, pH 0–12). Here, we describe the construction of Aho7c combinatorial libraries and their use for selection of binders by ribosome display.

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Acknowledgments

The authors thank all previous members of the laboratory who helped to develop this protocol. In memoriam of Ghislaine Béhar.

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Authors and Affiliations

  1. CRCINA, INSERM, CNRS, Université d’Angers, Université de Nantes, Nantes, France

    Valentina Kalichuk, Stanimir Kambarev, Ghislaine Béhar, Benjamin Chalopin, Axelle Renodon-Cornière, Barbara Mouratou & Frédéric Pecorari

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  1. Valentina Kalichuk
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  2. Stanimir Kambarev
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  3. Ghislaine Béhar
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  4. Benjamin Chalopin
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  5. Axelle Renodon-Cornière
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  6. Barbara Mouratou
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  7. Frédéric Pecorari
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Corresponding author

Correspondence to Frédéric Pecorari .

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Editors and Affiliations

  1. Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    Stefan Zielonka

  2. Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    Simon Krah

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Kalichuk, V. et al. (2020). Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins. In: Zielonka, S., Krah, S. (eds) Genotype Phenotype Coupling. Methods in Molecular Biology, vol 2070. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9853-1_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9853-1_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9852-4

  • Online ISBN: 978-1-4939-9853-1

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