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Genotype Phenotype Coupling

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Stefan Zielonka

    1. Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Simon Krah

    1. Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes an implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2070)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin

    • Nandi Zhou, Rongfeng Cai, Xuyan Han
    Pages 1-18

  3. Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins

    • Valentina Kalichuk, Stanimir Kambarev, Ghislaine Béhar, Benjamin Chalopin, Axelle Renodon-Cornière, Barbara Mouratou et al.
    Pages 19-41

  4. cDNA Display: A Stable and Simple Genotype–Phenotype Coupling Using a Cell-Free Translation System

    • Hidenao Arai, Shigefumi Kumachi, Naoto Nemoto
    Pages 43-56

  5. cDNA Display of Disulfide-Containing Peptide Library and In Vitro Evolution

    • Tai Kubo, Mohammed Naimuddin
    Pages 57-77

  6. Rapid Antigen and Antibody-Like Molecule Discovery by Staphylococcal Surface Display

    • Marco Cavallari
    Pages 79-94

  7. Restriction-Free Construction of a Phage-Presented Very Short Macrocyclic Peptide Library

    • Valentin Jakob, Saskia Helmsing, Michael Hust, Martin Empting
    Pages 95-113

  8. In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties

    • John Steven, Obinna C. Ubah, Magdalena Buschhaus, Marina Kovaleva, Laura Ferguson, Andrew J. Porter et al.
    Pages 115-142

  9. Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens

    • Esther V. Wenzel, Kristian D. R. Roth, Giulio Russo, Viola Fühner, Saskia Helmsing, André Frenzel et al.
    Pages 143-155

  10. Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning

    • Kathryn M. Frietze, Susan B. Core, Alexandria Linville, Bryce Chackerian, David S. Peabody
    Pages 157-171

  11. Isolation of Antigen-Specific VHH Single-Domain Antibodies by Combining Animal Immunization with Yeast Surface Display

    • Lukas Roth, Simon Krah, Janina Klemm, Ralf Günther, Lars Toleikis, Michael Busch et al.
    Pages 173-189

  12. Selection and Characterization of Anti-idiotypic Shark Antibody Domains

    • Doreen Könning, Stefan Zielonka, Anna Kaempffe, Sebastian Jäger, Harald Kolmar, Christian Schröter
    Pages 191-209

  13. Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression

    • Steffen C. Hinz, Adrian Elter, Julius Grzeschik, Jan Habermann, Bastian Becker, Harald Kolmar
    Pages 211-222

  14. Methods for Construction of Yeast Display Libraries of Four-Domain T-Cell Receptors

    • Flávio Sádio, Gerhard Stadlmayr, Katja Eibensteiner, Katharina Stadlbauer, Florian Rüker, Gordana Wozniak-Knopp
    Pages 223-248

  15. Isolation of Tailor-Made Antibody Fragments from Yeast-Displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting

    • Anna Kaempffe, Sebastian Jäger, Doreen Könning, Harald Kolmar, Christian Schröter
    Pages 249-266

  16. Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires

    • Sebastian Jäger, Simon Krah, Doreen Könning, Janis Rosskopf, Stephan Dickgiesser, Nicolas Rasche et al.
    Pages 267-287

  17. Rapid Generation of Chicken Immune Libraries for Yeast Surface Display

    • Jan P. Bogen, Julius Grzeschik, Simon Krah, Stefan Zielonka, Harald Kolmar
    Pages 289-302

  18. Ligand Engineering via Yeast Surface Display and Adherent Cell Panning

    • Lawrence A. Stern, Patrick S. Lown, Benjamin J. Hackel
    Pages 303-320

  19. Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping

    • Carlos A. Cruz-Teran, Kaitlyn Bacon, Balaji M. Rao
    Pages 321-334

  20. Chemical Modification of the Yeast Cell Surface Allows the Switch Between Display and Soluble Secretion of Full-Length Antibodies

    • Simon Krah, Ralf Günther, Stefan Becker, Stefan Zielonka, Laura Rhiel
    Pages 335-349
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Keywords

About this book

This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Genotype Phenotype Coupling aims to provide an overview of current technologies in this exciting and continuously evolving field. 

Editors and Affiliations

  • Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    Stefan Zielonka, Simon Krah

Bibliographic Information

  • Book Title: Genotype Phenotype Coupling

  • Book Subtitle: Methods and Protocols

  • Editors: Stefan Zielonka, Simon Krah

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9853-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9852-4Published: 18 October 2019

  • Softcover ISBN: 978-1-4939-9855-5Published: 18 October 2020

  • eBook ISBN: 978-1-4939-9853-1Published: 17 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 445

  • Number of Illustrations: 25 b/w illustrations, 61 illustrations in colour

  • Topics: Human Genetics, Protein Science

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