Abstract
A series of ligand-targeted nanosystems have been rapidly exploited to selectively deliver drug molecules to desired cell populations. The conjugation of protein ligands to the nanoparticle (NP) surface endows nanovehicles with active targeting properties. However, the nonspecific covalent coupling of protein ligands to nanocarriers may compromise the protein targeting due to the uncontrolled ligand orientation as well as the decline in ligand activity during linkage process. With this regard, biomimetic synthetic strategies are employed for the preparation of genetically engineered nanovesicles (GNV) from cellular plasma membrane with targeting moieties on the surface in a ligand-oriented manner. Herein, we introduce the biomimetic synthetic strategy and procedures for GNV preparation. This chapter may guide readers to design analogous NPs for cell-specific targeting by displaying particular protein probes (e.g., antibody, nanobody, and single-chain antibody) on the surface of GNVs.
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References
Chu C, Lin H, Liu H, Wang X, Wang J, Zhang P et al (2017) Tumor microenvironment-triggered supramolecular system as an in situ nanotheranostic generator for cancer phototherapy. Adv Mater 29:1605928
Zhang L, Gao S, Zhang F, Yang K, Ma Q, Zhu L (2014) Activatable hyaluronic acid nanoparticle as a theranostic agent for optical/photoacoustic image-guided photothermal therapy. ACS Nano 8:12250–12258
Cai W, Gao H, Chu C, Wang X, Wang J, Zhang P et al (2017) Engineering photo-theranostic nanoscale metal-organic frameworks for multi-modal imaging-guided cancer therapy. ACS Appl Mater Interfaces 9:2040–2051
Topete A, Alatorre-Meda M, Iglesias P, Villar-Alvarez EM, Barbosa S, Costoya JA et al (2014) Fluorescent drug-loaded, polymeric-based, branched gold nanoshells for localized multimodal therapy and imaging of tumoral cells. ACS Nano 8:2725–2738
Lin G, Zhang Y, Zhu C, Chu C, Shi Y, Pang X et al (2018) Photo-excitable hybrid nanocomposites for image-guided photo/TRAIL synergistic cancer therapy. Biomaterials 176:60–70
Wang J, Tao W, Chen X, Farokhzad OC, Liu G (2017) Emerging advances in nanotheranostics with intelligent bioresponsive systems. Theranostics 7:3915–3919
Ying M, Zhan C, Wang S, Yao B, Hu X, Song X et al (2016) Liposome-based systemic glioma-targeted drug delivery enabled by all-d peptides. ACS Appl Mater Interfaces 8:29977–29985
Mikhaylov G, Mikac U, Magaeva AA, Itin VI, Naiden EP, Psakhye I et al (2011) Ferri-liposomes as an MRI-visible drug-delivery system for targeting tumours and their microenvironment. Nat Nanotechnol 6:594–602
Lin G, Mi P, Chu C, Zhang J, Liu G (2016) Inorganic nanocarriers overcoming multidrug resistance for cancer theranostics. Adv Sci 3:1600134
Zhang P, Zhang L, Qin Z, Hua S, Guo Z, Chu C et al (2018) Genetically engineered liposome-like nanovesicles as active targeted transport platform. Adv Mater 30:1705350
Noble GT, Stefanick JF, Ashley JD, Kiziltepe T, Bilgicer B (2014) Ligand-targeted liposome design: challenges and fundamental considerations. Trends Biotechnol 32:32–45
Mazzucchelli S, Colombo M, Verderio P, Rozek E, Andreata F, Galbiati E et al (2013) Orientation-controlled conjugation of haloalkane dehalogenase fused homing peptides to multifunctional nanoparticles for the specific recognition of cancer cells. Angew Chem Int Ed Engl 52:3121–3125
Zhang P, Liu G, Chen X (2017) Nanobiotechnology: cell membrane-based delivery systems. Nano Today 13:7–9
Jang SC, Kim OY, Yoon CM, Choi DS, Roh TY, Park J et al (2013) Bioinspired exosome-mimetic nanovesicles for targeted delivery of chemotherapeutics to malignant tumors. ACS Nano 7:7698–7710
Zhang P, Chen Y, Zeng Y, Shen C, Li R, Guo Z et al (2015) Virus-mimetic nanovesicles as a versatile antigen-delivery system. Proc Natl Acad Sci U S A 112:E6129–E6138
Somiya M, Kuroda S (2015) Development of a virus-mimicking nanocarrier for drug delivery systems: the bio-nanocapsule. Adv Drug Deliv Rev 95:77–89
Wang D, Dong H, Li M, Cao Y, Yang F, Zhang K et al (2018) Erythrocyte-cancer hybrid membrane camouflaged hollow copper sulfide nanoparticles for prolonged circulation life and homotypic-targeting photothermal/chemotherapy of melanoma. ACS Nano 12:5241–5252
Hu CM, Zhang L, Aryal S, Cheung C, Fang RH, Zhang L (2011) Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform. Proc Natl Acad Sci U S A 108:10980–10985
Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F et al (2008) Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science 319:1244–1247
Wolfers J, Lozier A, Raposo G, Regnault A, Thery C, Masurier C et al (2001) Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming. Nat Med 7:297–303
Thery C, Zitvogel L, Amigorena S (2002) Exosomes: composition, biogenesis and function. Nat Rev Immunol 2:569–579
Yoo JW, Irvine DJ, Discher DE, Mitragotri S (2011) Bio-inspired, bioengineered and biomimetic drug delivery carriers. Nat Rev Drug Discov 10:521–535
Hutchens SA, Benson RS, Evans BR, O’Neill HM, Rawn CJ (2006) Biomimetic synthesis of calcium-deficient hydroxyapatite in a natural hydrogel. Biomaterials 27:4661–4670
Mantion A, Graf P, Florea I, Haase A, Thunemann AF, Masic A et al (2011) Biomimetic synthesis of chiral erbium-doped silver/peptide/silica core-shell nanoparticles (ESPN). Nanoscale 3:5168–5179
Zhao L, Wang J, Lv P, Liu G (2018) Biomimetic synthesis of nanovesicles for targeted drug delivery. Sci Bull 63:663–665
Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant Nos. 81371596, 81422023, 51273165, U1705281, and U1505221), the Major State Basic Research Development Program of China (Grant Nos. 2017YFA0205201 and 2018YFA0107301), the Program for New Century Excellent Talents in University (NCET-13-0502), and the Fundamental Research Funds for the Central Universities, China (20720150141 and 20720150206).
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Zhang, P., Chen, H., Liu, J., Liu, G. (2019). Genetically Engineered Plasma Membrane Nanovesicles for Cancer-Targeted Nanotheranostics. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_18
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DOI: https://doi.org/10.1007/978-1-4939-9769-5_18
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