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Using RNA Interference for Purinoceptor Knockdown In Vivo

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Purinergic Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2041))

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Abstract

RNA interference (RNAi) is a powerful post-transcriptional gene silencing (PTGS) induced by small double-stranded RNA (dsRNA). The method allows silencing of genes of interest by translation inhibition or by mRNA degradation. In this chapter, we provide a brief overview of the mechanisms involved in each step of gene silencing. A nonviral infusion of short siRNA into ventricular system of rats was used to study purinoceptor in the rat brain.

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Acknowledgments

The authors thank the Brazilian agencies Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support.

The authors declare no competing interests.

Author information

Authors and Affiliations

  1. Departamento de Neurologia e Neurocirurgia, Disciplina de Neurociência, Universidade Federal de São Paulo—UNIFESP, São Paulo, Brazil

    Rebeca Padrão Amorim & Maria Jose da Silva Fernandes

  2. Escola Paulista de Medicina, Universidade Federal de SÐo Paulo (EPM/UNIFESP), São Paulo, Brazil

    Maria Jose da Silva Fernandes

  3. Departamento de Genetica Médica, Escola de Ciências Médicas da Universidade de Campinas—UNICAMP, Campinas, São Paulo, Brazil

    Iscia Teresinha Lopes Cendes

Authors
  1. Rebeca Padrão Amorim
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  2. Iscia Teresinha Lopes Cendes
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  3. Maria Jose da Silva Fernandes
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Corresponding author

Correspondence to Maria Jose da Silva Fernandes .

Editor information

Editors and Affiliations

  1. BioMedical Research Institute of Murcia (IMIB), Clinical University Hospital Virgen de la Arrixaca, Murcia, Spain

    Pablo Pelegrín

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Amorim, R.P., Lopes Cendes, I.T., da Silva Fernandes, M.J. (2020). Using RNA Interference for Purinoceptor Knockdown In Vivo. In: Pelegrín, P. (eds) Purinergic Signaling. Methods in Molecular Biology, vol 2041. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9717-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9717-6_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9716-9

  • Online ISBN: 978-1-4939-9717-6

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