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Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates

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ADAMTS Proteases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2043))

Abstract

Here we describe the use of a decellularized ECM produced in vitro by BALB/c 3T3 fibroblasts for the identification of ADAMTS substrates. Seeding of ADAMTS protease-producing HEK cells on top of the cell-free ECM followed by analysis of the conditioned medium by liquid chromatography tandem mass spectrometry (LC-MS/MS), allows for screening of ADAMTS substrates without prior purification of full-length protease.

Ruth Chiquet-Ehrismann was deceased at the time of publication.

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Acknowledgments

This work was supported by a grant from The Swiss National Science Foundation (SNF, Project funding in biology and medicine (division III)) grant no. 31003A_156740.

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Author notes

  1. Rahel Schnellmann

    Present address: Department of Chemical and Biomolecular Engineering, The Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA

Authors and Affiliations

  1. Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

    Rahel Schnellmann & Ruth Chiquet-Ehrismann

  2. Faculty of Science, University of Basel, Basel, Switzerland

    Rahel Schnellmann & Ruth Chiquet-Ehrismann

Authors
  1. Rahel Schnellmann
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  2. Ruth Chiquet-Ehrismann
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Corresponding author

Correspondence to Rahel Schnellmann .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA

    Suneel S. Apte

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Schnellmann, R., Chiquet-Ehrismann, R. (2020). Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates. In: Apte, S. (eds) ADAMTS Proteases. Methods in Molecular Biology, vol 2043. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9698-8_23

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  • DOI: https://doi.org/10.1007/978-1-4939-9698-8_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9697-1

  • Online ISBN: 978-1-4939-9698-8

  • eBook Packages: Springer Protocols

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