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Embryonic Stem Cells and Gene Manipulation in Rat

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Rat Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2018))

Abstract

Transgenic technology in rats is increasingly important for the design and implementation of biological and physiological studies in the fields of neuroscience, pharmacology, and toxicology. Pluripotent embryonic stem cells (ESCs) are a useful tool for generation of gene-modified rats. During the last decade, not only foreign DNA introduction but also endogenous DNA modification has been successfully achieved with rat ESCs. Detailed protocols for establishment of bona fide rat ESCs and their use for production of gene-modified rats are described in this chapter.

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Author information

Authors and Affiliations

  1. Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, Japan

    Masumi Hirabayashi

  2. Genomic Sciences and Precision Medicine Center, Medical College of Wisconsin, Milwaukee, WI, USA

    Akiko Takizawa

  3. Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan

    Shinichi Hochi

Authors
  1. Masumi Hirabayashi
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  2. Akiko Takizawa
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  3. Shinichi Hochi
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Corresponding author

Correspondence to Masumi Hirabayashi .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, Rat Genome Database, Medical College of Wisconsin, Milwaukee, WI, USA

    G. Thomas Hayman

  2. Department of Biomedical Engineering, Rat Genome Database, Medical College of Wisconsin, Milwaukee, WI, USA

    Jennifer R. Smith

  3. Department of Physiology, Rat Genome Database, Genomic Sciences and Precision Medicine Center, Medical College of Wisconsin, Milwaukee, WI, USA

    Melinda R. Dwinell

  4. Department of Biomedical Engineering, Rat Genome Database, Medical College of Wisconsin, Milwaukee, WI, USA

    Mary Shimoyama

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Hirabayashi, M., Takizawa, A., Hochi, S. (2019). Embryonic Stem Cells and Gene Manipulation in Rat. In: Hayman, G., Smith, J., Dwinell, M., Shimoyama, M. (eds) Rat Genomics. Methods in Molecular Biology, vol 2018. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9581-3_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9581-3_5

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9580-6

  • Online ISBN: 978-1-4939-9581-3

  • eBook Packages: Springer Protocols

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