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Phloem pp 95–108Cite as

Live-Cell Imaging of Fluorescently Tagged Phloem Proteins with Confocal Microscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2014))

Abstract

Confocal laser scanning microscopy can enable observation of phloem cells in living tissues. Here we describe live imaging of phloem cells in the leaves and roots of Arabidopsis thaliana using fluorescently tagged proteins, either expressed in the vasculature using phloem specific promoters or constitutively expressed reference marker lines. Now, the majority of phloem cell types can be identified, allowing a precise cellular and subcellular localization of phloem proteins.

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Abbreviations

CC:

Companion cell

CFP:

Cyan fluorescent protein

GFP:

Green fluorescent protein

PPC:

Phloem parenchyma cell

SE:

Sieve element

YFP:

Yellow fluorescent protein

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Acknowledgments

The IJPB benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS) managed by the French National Research Agency under an “Investments for the Future” program (ref. ANR-11-IDEX-0003-02). We thank the Imaging and Cytology platform of the Plant Observatory (IJPB Institute, INRA Versailles-Grignon, France) for light microscopy observations. TC was supported by a PhD fellowship from Doctoral School ED145 “Sciences du Végétal,” Paris—Sud XI University.

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Authors and Affiliations

  1. UMR 1318, Institut Jean-Pierre Bourgin, INRA-AgroParisTech, CNRS, Université Paris-Saclay, Versailles Cedex, France

    Thibaud Cayla, Rozenn Le Hir & Sylvie Dinant

Authors
  1. Thibaud Cayla
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  2. Rozenn Le Hir
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  3. Sylvie Dinant
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Corresponding author

Correspondence to Sylvie Dinant .

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Editors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, China

    Johannes Liesche

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Cayla, T., Le Hir, R., Dinant, S. (2019). Live-Cell Imaging of Fluorescently Tagged Phloem Proteins with Confocal Microscopy. In: Liesche, J. (eds) Phloem. Methods in Molecular Biology, vol 2014. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9562-2_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9562-2_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9561-5

  • Online ISBN: 978-1-4939-9562-2

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