Abstract
Four-stranded G-quadruplexes consists of tracks of guanines that are stabilized by Hoogsteen base pairing. The formation of G-quadruplexes in genomic DNA contribute to numerous biological processes in vivo, including replication, transcription, and telomere maintenance. Here, we present a detailed method to detect the colocalization of G-quadruplexes with telomeres in vivo, using the BG4 antibody developed from Dr. Shankar Balasubramanian’s lab.
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Acknowledgments
We thank Dr. Shankar Balasubramanian (University of Cambridge, UK) for providing BG4 antibody (plasmid). This work was supported by Ministry of Science and Technology (2017YFC1001904), National Natural Science Foundation of China (91649102, 31771520, 21177091, 21647008, 31471293, 81501386, 81671054, 81771135), the Key Project of Tianjin Research Program of Application Foundation and Advanced Technology (15JCZDJC35100).
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Zhang, M., Liu, R., Wang, F. (2019). Telomere and G-Quadruplex Colocalization Analysis by Immunofluorescence Fluorescence In Situ Hybridization (IF-FISH). In: Balakrishnan, L., Stewart, J. (eds) DNA Repair. Methods in Molecular Biology, vol 1999. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9500-4_23
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DOI: https://doi.org/10.1007/978-1-4939-9500-4_23
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