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Metabolomics Analyses of Muscle Atrophy Induced by Hind Limb Unloading

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Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1996))

Abstract

Acute periods of contractile inactivity cause skeletal muscle atrophy along with profound alterations in tissue metabolism. Hind limb unloading via tail suspension is a commonly used rodent model of muscle atrophy. Here, we describe a sample preparation and LC-MS/MS approach for quantifying specific panels of acylcarnitines, amino acids, and organic acids in small (~8 mg) samples of atrophied mouse soleus following a period of hind limb unloading.

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Acknowledgments

We are grateful for the excellent technical assistance of the staff at the Vivarium and the Metabolomics core of the Sanford Burnham Prebys Medical Discovery Institute at Lake Nona (SBP-LN). The authors have no conflicts of interest to declare. This study was supported by funding from (a) the National Institutes of Health | National Institute on Aging (K01 AG044437) awarded to PMC and (b) National Institutes of Health Common Fund (U24 DK097209-03) awarded to the Southeast Center for Integrated Metabolomics (SECIM), a partnership between University of Florida and SBP-LN (SJG, Co-PI). The capability to perform the assays described in this methods chapter was transferred to the Translational Research Institute for Metabolism and Diabetes at Florida Hospital (Orlando, FL).

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Author notes

  1. Nidhi Kapoor

    Present address: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA

  2. Stephen J. Gardell

    Present address: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA

  3. Christopher Petucci

    Present address: Department of Medicine, Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

Authors and Affiliations

  1. Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA

    Stephen J. Gardell & Christopher Petucci

  2. Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA

    Nidhi Kapoor & Paul M. Coen

  3. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China

    Xiaolei Zhang

  4. Southeast Center for Integrated Metabolomics SECIM, Gainesville, FL, USA

    Christopher Petucci

Authors
  1. Stephen J. Gardell
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  2. Xiaolei Zhang
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  3. Nidhi Kapoor
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  4. Christopher Petucci
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  5. Paul M. Coen
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Corresponding author

Correspondence to Paul M. Coen .

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Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA

    Sanjoy K. Bhattacharya

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Gardell, S.J., Zhang, X., Kapoor, N., Petucci, C., Coen, P.M. (2019). Metabolomics Analyses of Muscle Atrophy Induced by Hind Limb Unloading. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9488-5_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9487-8

  • Online ISBN: 978-1-4939-9488-5

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