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Redox-Mediated Signal Transduction pp 93–102Cite as

Applications of Electron Paramagnetic Resonance (EPR) Spectroscopy in the Study of Oxidative Stress in Biological Systems

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1990))

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is the most direct and powerful method for the detection and identification of free radicals and other molecules with unpaired electrons. Such species are generated by and are crucial to mechanisms of oxidative stress in biological systems, and EPR spectroscopy offers a unique ability to detect, identify, and quantitate free radicals to aid our understanding of the role of these species in oxidative stress. This chapter outlines the application of EPR spectroscopy to the study of important reactive oxygen and nitrogen molecules in biological systems including their detection in vivo.

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References

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Author information

Authors and Affiliations

  1. Faculty of Medicine and Dentistry, Institute of Translational and Stratified Medicine, School of Biomedical Sciences, University of Plymouth, Plymouth, UK

    Simon K. Jackson

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  1. Simon K. Jackson
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Correspondence to Simon K. Jackson .

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Editors and Affiliations

  1. Department of Applied Sciences, University of the West of England, Bristol, UK

    John T. Hancock

  2. Department of Applied Sciences, University of the West of England, Bristol, UK

    Myra E. Conway

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Jackson, S.K. (2019). Applications of Electron Paramagnetic Resonance (EPR) Spectroscopy in the Study of Oxidative Stress in Biological Systems. In: Hancock, J., Conway, M. (eds) Redox-Mediated Signal Transduction. Methods in Molecular Biology, vol 1990. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9463-2_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9461-8

  • Online ISBN: 978-1-4939-9463-2

  • eBook Packages: Springer Protocols

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