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NADPH Oxidases pp 283–299Cite as

Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1982))

Abstract

Quantitative aspects of extracellular H2O2 signaling in animals, such as its spatiotemporal dynamics within tissues, remain little understood. Here we detail an optimized, experimental setup for measuring the dynamics and physiological consequences of extracellular H2O2 application to live tissues by intravital biosensor imaging in zebrafish larvae.

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Acknowledgments

Research was supported by the NIH/NIGMS grant R01GM099970, an American Asthma Foundation Scholar award to PN, the MSKCC Functional Genome Initiative, and in part through the NIH/NCI Cancer Center Support grant P30CA008748.

Author information

Authors and Affiliations

  1. Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Mark Jelcic & Philipp Niethammer

  2. Louis V. Gerstner, Jr. Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Mark Jelcic

  3. Faculty of Medicine, Department of Physiology, Semmelweis University, Budapest, Hungary

    Balázs Enyedi

  4. MTA-SE Lendület Tissue Damage Research Group, Hungarian Academy of Sciences and Semmelweis University, Budapest, Hungary

    Balázs Enyedi

Authors
  1. Mark Jelcic
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  2. Balázs Enyedi
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  3. Philipp Niethammer
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Corresponding author

Correspondence to Philipp Niethammer .

Editor information

Editors and Affiliations

  1. Conway Institute, School of Medicine, University College Dublin, Dublin, Ireland

    Ulla G. Knaus

  2. Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Thomas L. Leto

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Jelcic, M., Enyedi, B., Niethammer, P. (2019). Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae. In: Knaus, U., Leto, T. (eds) NADPH Oxidases. Methods in Molecular Biology, vol 1982. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9424-3_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9424-3_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9423-6

  • Online ISBN: 978-1-4939-9424-3

  • eBook Packages: Springer Protocols

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