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Deciphering the Neural Crest Contribution to Cephalic Development with Avian Embryos

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Neural Crest Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1976))

Abstract

For decades, the quail-chick system has been a gold standard approach to track cells and their progenies over complex morphogenetic movements and long-range migrations as well as to unravel their dialogue and interplays in varied processes of cell induction. More specifically, this model became decisive for the systematic explorations of the neural crest and its lineages and allowed a tremendous stride in understanding the wealth and complexity of this fascinating cell population. Much of our knowledge on craniofacial morphogenesis and vertebrate organogenesis was first gained in avian chimeras and later extended to mammalian models and humans. In addition, this system permits tissue and gene manipulations to be performed at once in the same cell population. Through the use of in ovo electroporation, this model became tractable for functional genomics, hence being even more resourceful for functional studies. Due to the ease of access and the possibility to combine micromanipulation of tissue anlagen and gene expression, this model offers the prospect of decrypting instructive versus permissive tissue interactions, to identify and crack the molecular codes underlying cell positioning and differentiation, with an unparalleled spatiotemporal accuracy.

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Acknowledgments

This work is supported by the Centre National de la Recherche Scientifique (CNRS) and the Fondation pour la Recherche Médicale (FRM-, DEQ20170839116 grant). MA and ZV are recipients of fellowships from the FRM (FRM-MedicoSci program) and the National Institutes of Health (Richard A. Schneider’s lab, University of California, San Francisco, USA), respectively.

Author information

Author notes

  1. Zuzana Vavrusova

    Present address: Department of Orthopaedic Surgery, UCSF School of Medicine, San Francisco, CA, USA

  2. Moussab Alrajeh and Zuzana Vavrusova contributed equally to this work.

Authors and Affiliations

  1. Institut des Neurosciences Paris-Saclay, Neuro-PSI CNRS UMR 9197, Gif-sur-Yvette, France

    Moussab Alrajeh, Zuzana Vavrusova & Sophie E. Creuzet

Authors
  1. Moussab Alrajeh
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  2. Zuzana Vavrusova
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  3. Sophie E. Creuzet
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Corresponding author

Correspondence to Sophie E. Creuzet .

Editor information

Editors and Affiliations

  1. Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, SA, Australia

    Quenten Schwarz

  2. Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, SA, Australia

    Sophie Wiszniak

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Alrajeh, M., Vavrusova, Z., Creuzet, S.E. (2019). Deciphering the Neural Crest Contribution to Cephalic Development with Avian Embryos. In: Schwarz, Q., Wiszniak, S. (eds) Neural Crest Cells. Methods in Molecular Biology, vol 1976. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9412-0_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9412-0_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9411-3

  • Online ISBN: 978-1-4939-9412-0

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