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Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions

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Proteomics for Biomarker Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1959))

Abstract

Characterization of protein structure modifications is an important field in mass spectrometry (MS)-based proteomics. Here, we describe a process to quickly and reliably identify a mass change in a targeted protein sequence by top-down mass spectrometry (TD MS) using electron transfer dissociation (ETD). The step-by-step procedure describes how to develop a TD MS method for data acquisition as well as the data analysis process. The described TD MS workflow utilizes diagnostic ions to characterize an unknown sample in a few hours.

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References

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Authors and Affiliations

  1. Clinical Proteomics and Chemistry Group, Faculty of Medicine, University of Geneva, Geneva, Switzerland

    Didia Coelho Graça, Alexander Scherl, Denis Hochstrasser & Pierre Lescuyer

  2. Department of Genetic, Laboratory Medicine and Pathology, Geneva University Hospitals, Geneva, Switzerland

    Didia Coelho Graça, Alexander Scherl, Lorella Clerici, Kaveh Samii, Denis Hochstrasser & Pierre Lescuyer

  3. Bruker Daltonics, Bremen, Germany

    Ralf Hartmer

  4. Beuth Hochschule für Technik Berlin, Berlin, Germany

    Wolfgang Jabs

  5. Division of Hematology, Geneva University Hospitals, Geneva, Switzerland

    Kaveh Samii

  6. Spectroswiss, Lausanne, Switzerland

    Yury O. Tsybin

Authors
  1. Didia Coelho Graça
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  2. Ralf Hartmer
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  3. Wolfgang Jabs
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  4. Alexander Scherl
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  5. Lorella Clerici
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  6. Kaveh Samii
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  7. Yury O. Tsybin
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  8. Denis Hochstrasser
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  9. Pierre Lescuyer
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Corresponding author

Correspondence to Didia Coelho Graça .

Editor information

Editors and Affiliations

  1. Université Grenoble Alpes, CEA, Inserm, BGE U1038, Grenoble, France

    Virginie Brun

  2. Université Grenoble Alpes, CEA, Inserm, BGE U1038, Grenoble, France

    Yohann Couté

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Coelho Graça, D. et al. (2019). Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions. In: Brun, V., Couté, Y. (eds) Proteomics for Biomarker Discovery. Methods in Molecular Biology, vol 1959. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9164-8_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9164-8_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9163-1

  • Online ISBN: 978-1-4939-9164-8

  • eBook Packages: Springer Protocols

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