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T. cruzi Infection pp 239–246Cite as

Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi Trans-sialidase

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1955))

Abstract

The trans-sialidase (TS), a virulence factor expressed on the surface of Trypanosoma cruzi, the agent of Chagas disease, is an enzyme that transfers sialic acids between glycoconjugates. In humans and most tested mammals, the onset of the chronic phase of T. cruzi infection correlates with the elicitation of antibodies directed to the TS catalytic domain, which inhibit the sialyl residues transfer reaction in vitro and in vivo. The method described here, termed trans-sialidase inhibition assay (TIA), enables the detection of TS-neutralizing antibodies in serum samples of different mammalian species, without the use of conjugated secondary reagents. The high specificity and exquisite sensitivity displayed by the TIA allow to overcome the limitations of routinely used Chagas disease serodiagnostic assays.

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Authors and Affiliations

  1. Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Buenos Aires, Argentina

    María Susana Leguizamón

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    María Susana Leguizamón

Authors
  1. María Susana Leguizamón
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Correspondence to María Susana Leguizamón .

Editor information

Editors and Affiliations

  1. INGEBI-CONICET, Buenos Aires, Argentina

    Karina Andrea Gómez

  2. UNSAM-CONICET, IIB-INTECH, San Martin, Buenos Aires, Argentina

    Carlos Andrés Buscaglia

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Leguizamón, M.S. (2019). Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi Trans-sialidase. In: Gómez, K., Buscaglia, C. (eds) T. cruzi Infection. Methods in Molecular Biology, vol 1955. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9148-8_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9148-8_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9147-1

  • Online ISBN: 978-1-4939-9148-8

  • eBook Packages: Springer Protocols

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