Abstract
The posttranslational modifications of histones and miRNAs are key epigenetic mechanisms participating in the development, growth, and reproduction of plants. Recently, coordination between these two mechanisms has been demonstrated; each mechanism can be controlled by the other for the regulation of several biological processes. For example, the acetylation of histone H3, a key modification for chromatin remodeling and gene activation, has been linked to the actions of miRNA. In this work, we describe a method for the isolation and immunodetection of two posttranslational modifications in the residues of lysine 9 and 27 of H3, which have been associated with long miRNAs in plants.
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Acknowledgments
This work was supported by grants from Consejo Nacional de Ciencia y Tecnología (CONACYT) to CD (CB2016-285898 and FC2016-1515) and a CONACYT scholarship to SHC (271240).
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Hernández-Castellano, S., De-la-Peña, C. (2019). Detection of Histone Modifications Associated with miRNAs. In: de Folter, S. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 1932. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9042-9_25
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DOI: https://doi.org/10.1007/978-1-4939-9042-9_25
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