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Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes

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Calcium Signalling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1925))

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Abstract

Calcium (Ca2+) is a key player in cardiomyocyte homeostasis, and its roles span from excitation-contraction coupling to metabolic and structural signaling. Alterations in the function or expression of Ca2+-handling proteins are common findings in failing cardiomyocytes, which have been linked to impaired contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca2+ handling in cardiomyocytes represents a central method in experimental molecular cardiology.

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Acknowledgments

The authors are grateful to Dr. Giulia Borile for her theoretical and technical help to develop and improve this protocol.

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Authors and Affiliations

  1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Antonio Campo & Marco Mongillo

  2. Venetian Institute of Molecular Medicine, Padua, Italy

    Marco Mongillo

  3. Neuroscience Institute, Italian National Research Council (CNR), Padua, Italy

    Marco Mongillo

Authors
  1. Antonio Campo
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  2. Marco Mongillo
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Corresponding author

Correspondence to Marco Mongillo .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Anna Raffaello

  2. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Denis Vecellio Reane

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Campo, A., Mongillo, M. (2019). Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes. In: Raffaello, A., Vecellio Reane, D. (eds) Calcium Signalling. Methods in Molecular Biology, vol 1925. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9018-4_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9018-4_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9017-7

  • Online ISBN: 978-1-4939-9018-4

  • eBook Packages: Springer Protocols

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