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A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning

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Human Monoclonal Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1904))

Abstract

Panning is a common process used for antibody selection from phage antibody libraries. There are several methods developed for a similar purpose, namely streptavidin mass spectrometry immunoassay (MSIA™) Disposable Automation Research Tips, magnetic beads, polystyrene immunotubes, and microtiter plate. The advantage of using a magnetic particle processor system is the ability to carry out phage display panning against multiple target antigens simultaneously in parallel. The system carries out the panning procedure using magnetic nanoparticles in microtiter plates. The entire incubation, wash, and elution process is then automated in this setup. The system also allows customization for the introduction of different panning stringencies. The nature of the biopanning process coupled with the limitation of the system means that minimal human intervention is required for the infection and phage packaging stage. However, the process still allows for rapid and reproducible antibody generation to be carried out.

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Acknowledgments

The authors would like to acknowledge the support from the Malaysian Ministry of Education under the Higher Institution Centre of Excellence (HICoE) Grant (Grant no. 311/CIPPM/44001005).

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Authors and Affiliations

  1. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia

    Angela Chiew Wen Ch’ng, Azimah Ahmad & Theam Soon Lim

  2. Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany

    Zoltán Konthur

  3. Analytical Biochemistry Research Centre, Universiti Sains Malaysia, Penang, Malaysia

    Theam Soon Lim

Authors
  1. Angela Chiew Wen Ch’ng
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  2. Azimah Ahmad
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  3. Zoltán Konthur
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  4. Theam Soon Lim
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Corresponding author

Correspondence to Theam Soon Lim .

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Editors and Affiliations

  1. Department of Pathology, The Hebrew University, Hadassah Medical School, Jerusalem, Israel

    Michael Steinitz

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Ch’ng, A.C.W., Ahmad, A., Konthur, Z., Lim, T.S. (2019). A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning. In: Steinitz, M. (eds) Human Monoclonal Antibodies. Methods in Molecular Biology, vol 1904. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8958-4_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8958-4_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8957-7

  • Online ISBN: 978-1-4939-8958-4

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