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The Hippo Pathway pp 27–42Cite as

Drosophila Genetics: The Power of Genetic Mosaic Approaches

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

Abstract

Drosophila melanogaster has been a central player in the discovery of the Hippo pathway and in understanding its in vivo functions. From a technique standpoint, the Flp-FRT system for the generation of genetic mosaics has been a principle tool. It has broadly been used in the discovery of Hippo pathway members in mutagenesis screens, in the analysis of target gene expression, and in genetic epistasis. Here we briefly introduce this tool, summarize its use in the Hippo pathway field, and provide a protocol for the generation of Flp-FRT clones in imaginal discs with dissection and staining for reporter gene expression to characterize candidate Hippo pathway genes.

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Authors and Affiliations

  1. Department of Biological Sciences, Sam Houston State University, Huntsville, TX, USA

    Mardelle Atkins

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  1. Mardelle Atkins
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Correspondence to Mardelle Atkins .

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Editors and Affiliations

  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

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Atkins, M. (2019). Drosophila Genetics: The Power of Genetic Mosaic Approaches. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8909-6

  • Online ISBN: 978-1-4939-8910-2

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